FastQCFastQC Report
Fri 10 Feb 2017
SRR3556590.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556590.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3984435
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318180.7985573864299456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194850.4890279299323492No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158840.3986512516831119No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA124310.3119890273024908No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT98700.24771391677866497No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT81930.20562513882143893No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA75490.18946224496070332No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68170.17109075690781755No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG65830.16521790416959997No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC57790.1450393845049549No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG56920.14285588797407914No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55800.1400449499113425No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT50190.12596516193638496No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA44800.11243752250946494No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG40610.10192160243547704No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG13750.059.2789231
GTATAAC11300.044.7530821
TAACGCA11300.044.7519574
TATCACG7600.044.6203352
TAGTACT18800.043.987425
ATAGTAC18950.043.0113334
GCATAGT20100.041.734442
TACAACG7150.041.604042
AGATTAC20750.041.0005342
CATAGTA23350.034.906413
GATTACT24950.034.5755423
TCACGCA10200.033.8297924
ATAACGC17700.032.603933
ACAACGC9450.031.478193
AAACGCA17800.030.0811215
CATGGGC134550.029.9790254
GTATCAC14950.029.4491631
GGACCGT14150.029.431556
TACTGGT25500.029.3969272
CATGGGG113950.029.3422574
Produced by FastQC (version 0.11.5)