FastQCFastQC Report
Fri 10 Feb 2017
SRR3556579.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556579.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2100022
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163500.7785632721942913No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90470.43080501061417453No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87950.4188051363271433No Hit
TGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGAGCGCTAG47870.22794999290483625No Hit
GTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGGGAAGATAG43740.2082835322677572No Hit
GTCTACAGCATGGTGAGAGCCCGTACACGTTCGGAGGGGGGACCAAGCTG43650.20785496532893466No Hit
ACTTATTACTGTCTACAGCATGGTGAGAGCCCGTACACGTTCGGAGGGGG40750.19404558618909706No Hit
GTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCAT39560.18837897888688784No Hit
ACATTGTACAGTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGT39200.1866647111315977No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA38720.18437902079121077No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA32690.1556650358901002No Hit
TCCCTGCACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT32360.15409362378108418RNA PCR Primer, Index 26 (95% over 21bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT32330.15395076813481No Hit
GGTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGC32080.1527603044158585No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31140.1482841608326008No Hit
GTACATGGGCCATCTCATCCCTGCACATCTCCGAGCCCACGAGACTCCTG29850.14214136804281097No Hit
GATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCACCCTCACGTT29630.14109375997013365No Hit
GCTGTAGACAGTAATAAGTTGCTGTATCGTCAGACTCCAGGCTGCTGATG29060.1383795026909242No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT28920.13771284300831135No Hit
GTAATAAGTTGCTGTATCGTCAGACTCCAGGCTGCTGATGGTTAGAGAAT28860.13742713171576298No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT27330.1301414937557797No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA26990.12852246309800563No Hit
CTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCTGCTCAT26430.12585582436755424No Hit
CCGCAACAGTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGG26030.12395108241723182No Hit
GTGAGAGCCCGTACACGTTCGGAGGGGGGACCAAGCTGGAAATAAAACGG25190.11995112432155472No Hit
TCTCATCCCTGCACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTAT25180.11990350577279667No Hit
ACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCTGTCCTGATCA25080.11942732028521606No Hit
GTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGTCACCAGGTGT25050.11928446463894188No Hit
CTCTAACACTCATTCCTGTTGAAGCTCTTGACAATGGGTGAAGTTGATGT24540.11685591865228079No Hit
GGTATAGGTCTCTCCGGAGTTCCATTCCTCCTCTGTCACAGTCAGGATGC24400.11618925896966793No Hit
GCACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT24190.11518926944574866RNA PCR Primer, Index 26 (100% over 24bp)
CTGCACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT24160.11504641379947449RNA PCR Primer, Index 26 (100% over 22bp)
TCCTTGGTCAACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCT23860.11361785733673266No Hit
GTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCA23760.11314167184915205No Hit
GTATCAACGCAGAGTACATGGGCCATCTCATCCCTGCACATCTCCGAGCC23760.11314167184915205No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG23350.11118931135007157No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC23310.11099883715503932No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT22700.10809410568079762No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT22460.10695126051060418No Hit
GTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTT22300.10618936373047522No Hit
GAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAACACTCA22260.10599888953544297No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22230.10585603388916878No Hit
GTTAACATCTGGAGGTGCCTCAGTCGTGTGCTTCTTGAACAACTTCTACC21890.10423700323139472No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA21800.10380843629257216No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC21040.10018942658695956No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG4350.068.390681
ATAGTAC5050.058.9051784
AGATTAC6400.052.9870452
TAACGCA4000.052.057454
CATAGTA5900.050.4188353
TACAACG2250.050.239572
GTATAAC4350.047.8734781
TAGTACT6250.047.5953835
GCATAGT6400.047.4094622
TCACGCA3150.047.2176444
ATCTACG2550.044.3290332
GACCGTT2550.044.3290337
TTAGGAC3200.042.7614753
GTACAAC2650.042.66031
TATCTAC2650.042.66031
GATTACT8100.042.6008033
GTATCAC3650.042.3834881
TATCACG3900.038.1373252
CGCGAGT801.19849254E-437.183892
ATTACTT9300.037.1039244
Produced by FastQC (version 0.11.5)