FastQCFastQC Report
Fri 10 Feb 2017
SRR3556576.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556576.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1477490
Sequences flagged as poor quality0
Sequence length125
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180371.2207866043086586No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108360.7334059790590799No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95300.6450128258059277No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA58820.39810760140508566No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA50330.3406452835552186No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT46260.3130985658109361No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT44640.3021340245957671No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT44550.30152488341714667No Hit
CTGCACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT43690.2957042010436619RNA PCR Primer, Index 46 (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42640.2885975539597561No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA38160.25827585973509126No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35970.24345342438865913No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG35760.242032094971878No Hit
GCACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT35730.2418290479123378RNA PCR Primer, Index 46 (95% over 23bp)
GTACATGGGCCATCTCATCCCTGCACATCTCCGAGCCCACGAGACTAAGG26540.17962896533986694No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA25480.17245463590278107No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG25460.17231927119642096No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC23180.15688769467136834No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA22420.15174383582968412No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC21460.14524632992439881No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA20890.14138843579313568No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20450.1384104122532132No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20000.13536470636011075No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19220.1300854828120664No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG18850.12758123574440436No Hit
GAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAA18700.12656600044670352No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC17990.12176055337091961No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC17740.12006849454141821No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT17440.11803802394601656No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT17280.11695510629513566No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA17060.11546609452517445No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC17010.11512768275927417No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA16080.10883322391352904No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA15770.10673507096494732No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC15460.1046369180163656No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT15300.1035540003654847No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG7100.085.4746861
TACAACG3650.079.8700942
GTACAAC4300.071.949971
GTATAAC4950.069.713771
ATAGTAC9150.067.6228264
TAGTACT9500.064.505195
GCATAGT10000.061.2799262
TAACGCA6650.058.153084
GAGTTCT9600.057.6378061
ACAACGC5200.056.0626683
AGATTAC11550.055.6317562
CATAGTA11250.054.4710463
AGTGTAC6600.048.6777883
AGTTCTT11400.048.013572
TAAACGC4550.047.073034
GGTTTCA8500.046.8977051
ATCTACG3450.046.5613632
ATAACGC8050.046.5613633
GTGTACT7400.045.827294
GATTACT14100.045.148753
Produced by FastQC (version 0.11.5)