FastQCFastQC Report
Fri 10 Feb 2017
SRR3556571.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556571.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1243832
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109330.8789772252201261No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA81580.6558763562924896No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT72560.5833585243023174No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71230.5726657619356955No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT64680.520005917197821No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61890.4975752352407721No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG42640.34281156940808727No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG41480.3334855511033645No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT36240.2913576753130648No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC36060.2899105345416423No Hit
CTGCACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT35900.28862418718926675TruSeq Adapter, Index 11 (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA35050.28179046687977155No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30090.2419136989561291No Hit
GCACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT29650.23837624373709634TruSeq Adapter, Index 11 (95% over 23bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC28340.2278442747895214No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA27790.22342245576573044No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG25580.20565478296104295No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23170.18627917596588606No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC22610.18177696023257162No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA21930.17630998398497547No Hit
GTACATGGGCCATCTCATCCCTGCACATCTCCGAGCCCACGAGACCGTAC19630.15781874079457678No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA19570.15733636053743594No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT19340.15548723621839605No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC18520.1488947060374713No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC18040.14503566398034462No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG16990.13659400948038No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA15730.12646402408042245No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA15280.12284617215186616No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT14800.11898713009473948No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC14680.1180223695804578No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT14670.11794197287093434No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA14660.11786157616141087No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC14420.11593205513284752No Hit
GAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAA14390.1156908650042771No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14160.11384174068523724No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG14000.11255539333286167No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13860.11142983939953306No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT13780.11078666572334527No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC13520.10869635127573499No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC13300.10692762366621858No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC13150.10572167302336649No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG6150.071.58681
GTATAAC4900.065.565291
TACAACG1950.064.070922
TAGTACT8000.058.7483945
ATAGTAC8000.057.2610934
TAACGCA6000.056.517444
AGATTAC8200.055.8667222
TATCACG2700.055.0874372
GGTTTCA7050.054.0091251
ATAACGC6650.052.784543
GCATAGT8550.052.18812
CATAGTA8950.050.5204123
AGTGTAC6350.049.656773
TCACGCA3150.047.2159044
GATTACT9850.045.9043353
TCAAACG1900.043.8383
GAGTTCT7600.042.2723581
TTCAACG9150.042.2621044
GTGTACT7900.042.1715774
ATCTACG2300.041.3874322
Produced by FastQC (version 0.11.5)