FastQCFastQC Report
Fri 10 Feb 2017
SRR3556565.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556565.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989236
Sequences flagged as poor quality0
Sequence length125
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119941.212450820633297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73640.7444128600253125No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69120.6987210332013797No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA66750.6747631505525477No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT59310.5995535948954547No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT48200.4872447019720269No Hit
CTGCACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT43200.43670064575086226TruSeq Adapter, Index 11 (95% over 21bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37700.3811021839075812No Hit
GCACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT35930.3632095880052889TruSeq Adapter, Index 11 (95% over 23bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG35580.3596715040698074No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA33910.34278978929193843No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT31810.3215612856790493No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG31750.3209547570043953No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA30850.3118568268845857No Hit
GTACATGGGCCATCTCATCCCTGCACATCTCCGAGCCCACGAGACCGTAC30450.30781330238689253No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27120.27415096094359687No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC22080.22320255227266292No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG20950.21177959556667975No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC20930.21157741934179508No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC18310.18509233388190482No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16470.16649212119251625No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA16390.16568341629297761No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA16160.16335838970680402No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT15540.15709092673537964No Hit
GAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAA15510.15678766239805264No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC14470.1462744987040504No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA14080.14233206231879955No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT13690.13838962593354873No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13460.13606459934737516No Hit
TCCCTGCACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT12940.13080801750037402No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12880.13020148882572005No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA12540.12676449300268086No Hit
GTATCAACGCAGAGTACATGGGCCATCTCATCCCTGCACATCTCCGAGCC12440.12575361187825757No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC12170.12302423284231469No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA11990.12120464681835276No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG11840.11968832513171782No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA11810.11938506079439083No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11590.11716112232065957No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC11500.11625132930867862No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA11490.11615024119623629No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA10970.11089365934923516No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA10920.11038821878702353No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC10740.1085686327630616No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10720.10836645653817695No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAACACAGGTGGAAAA10530.10644578240177269No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC10200.10310987469117582No Hit
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA10170.10280661035384883No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT9990.1009870243298869No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG9970.10078484810500225No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG2400.071.893572
CGCATAG5050.067.15971
GTATAAC2700.066.112371
GTACAAC2750.062.7466551
ATAGTAC5750.060.012474
AGATTAC7550.059.8921552
TAGCGAG300.004237753359.495129
GGTTTCA4750.057.6221541
GCATAGT6200.054.6998942
TAGTACT6450.054.4218945
CATAGTA6250.054.2622953
TCACGCA1900.053.232474
GCAAGTA709.141877E-750.9983982
ACGCATA2350.050.6392631
AGTGTAC3800.048.5379453
TAACGCA4350.046.501934
GAGTTCT5400.046.2786641
GTCGACA653.52154E-545.765488
GATTACT10150.045.136513
CATGGGT15100.044.9168474
Produced by FastQC (version 0.11.5)