##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3556559.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 978957 Sequences flagged as poor quality 0 Sequence length 125 %GC 40 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09576007934976 33.0 32.0 34.0 30.0 34.0 2 32.105670627004045 33.0 32.0 34.0 30.0 34.0 3 32.14621684098484 33.0 32.0 34.0 30.0 34.0 4 32.02550060932196 33.0 32.0 34.0 30.0 34.0 5 32.055460045742564 33.0 32.0 34.0 30.0 34.0 6 35.20907762036535 38.0 35.0 38.0 29.0 38.0 7 35.59170321066196 38.0 36.0 38.0 30.0 38.0 8 35.8781580804877 38.0 37.0 38.0 31.0 38.0 9 36.04445751958462 38.0 37.0 38.0 31.0 38.0 10-11 35.984272036463295 38.0 37.0 38.0 31.0 38.0 12-13 36.024852470537525 38.0 37.0 38.0 31.5 38.0 14-15 36.15861830499195 38.0 37.0 38.0 32.5 38.0 16-17 36.145518648929425 38.0 37.0 38.0 33.0 38.0 18-19 36.18157641244712 38.0 37.0 38.0 33.0 38.0 20-21 36.05684876863846 38.0 37.0 38.0 31.5 38.0 22-23 36.05174282425071 38.0 37.0 38.0 32.0 38.0 24-25 36.009465175692085 38.0 37.0 38.0 32.0 38.0 26-27 35.89136346131649 38.0 37.0 38.0 31.0 38.0 28-29 35.75126742032592 38.0 37.0 38.0 30.0 38.0 30-31 35.44377587575348 38.0 36.5 38.0 28.5 38.0 32-33 35.08504459337846 38.0 36.0 38.0 26.5 38.0 34-35 34.62514032792043 38.0 35.5 38.0 24.5 38.0 36-37 34.167071689563485 38.0 35.0 38.0 16.0 38.0 38-39 33.80119709037271 38.0 34.0 38.0 15.0 38.0 40-41 33.40214585523164 38.0 33.5 38.0 15.0 38.0 42-43 32.921915875773905 38.0 32.0 38.0 15.0 38.0 44-45 32.27686609319919 38.0 28.0 38.0 15.0 38.0 46-47 31.602367111119285 38.0 25.5 38.0 15.0 38.0 48-49 30.968452649094903 38.0 24.5 38.0 15.0 38.0 50-51 30.463172539754044 37.5 20.0 38.0 15.0 38.0 52-53 30.06084485835435 37.0 15.5 38.0 15.0 38.0 54-55 29.692698453558226 37.0 15.0 38.0 15.0 38.0 56-57 29.40559033747141 36.0 15.0 38.0 15.0 38.0 58-59 29.19800563252523 36.0 15.0 38.0 15.0 38.0 60-61 29.027624298105025 36.0 15.0 38.0 15.0 38.0 62-63 28.858561203403212 35.5 15.0 38.0 14.0 38.0 64-65 28.655331643780066 35.0 15.0 38.0 14.0 38.0 66-67 28.436974249124322 35.0 15.0 38.0 14.0 38.0 68-69 28.200761116167513 34.0 15.0 38.0 14.0 38.0 70-71 27.963787479940386 34.0 15.0 38.0 14.0 38.0 72-73 27.717158669890505 34.0 14.5 38.0 14.0 38.0 74-75 27.525874987358996 33.0 14.0 38.0 14.0 38.0 76-77 27.341213148279238 33.0 14.0 38.0 14.0 38.0 78-79 27.160989195643936 31.5 14.0 38.0 14.0 38.0 80-81 27.02383250745436 31.0 14.0 38.0 13.5 38.0 82-83 26.84135309313892 30.5 14.0 38.0 13.0 38.0 84-85 26.7059896399944 29.0 14.0 38.0 13.0 38.0 86-87 26.572645683109677 29.0 14.0 38.0 13.0 38.0 88-89 26.414892073911318 27.5 14.0 38.0 13.0 38.0 90-91 26.22633067642399 26.5 14.0 38.0 13.0 38.0 92-93 26.148109671824194 25.5 14.0 38.0 13.0 38.0 94-95 26.060837197139406 25.0 14.0 38.0 13.0 38.0 96-97 25.95328088976329 24.5 14.0 38.0 13.0 38.0 98-99 25.844828730986144 24.0 14.0 38.0 13.0 38.0 100-101 25.700108380654104 24.0 14.0 38.0 13.0 38.0 102-103 25.543111188744756 23.5 14.0 38.0 13.0 38.0 104-105 25.424965550070127 23.0 14.0 38.0 13.0 38.0 106-107 25.299483021215437 23.0 14.0 38.0 13.0 38.0 108-109 25.086353639638922 23.0 14.0 38.0 13.0 38.0 110-111 24.963782372463754 23.0 14.0 38.0 13.0 38.0 112-113 24.82153455156866 23.0 14.0 38.0 13.0 38.0 114-115 24.714751005406775 23.0 14.0 38.0 13.0 38.0 116-117 24.487852377581447 23.0 14.0 38.0 13.0 38.0 118-119 24.34343745435193 23.0 14.0 38.0 13.0 38.0 120-121 24.295943539910333 23.0 14.0 38.0 13.0 38.0 122-123 24.195622483929323 23.0 14.0 37.0 13.0 38.0 124-125 23.524386157921136 22.0 14.0 35.5 13.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.032588946456051815 1101 2 0.2473922942814184 1101 3 0.05885441377134981 1101 4 0.2188592571383765 1101 5 0.2475773193434847 1101 6 0.3395714524363669 1101 7 0.23300647732848745 1101 8 0.16774431127218037 1101 9 0.2015248846438098 1101 10-11 -0.0019223370587084787 1101 12-13 0.12325597719605952 1101 14-15 0.028560426841444553 1101 16-17 -0.10316165177584224 1101 18-19 -0.1856579039037527 1101 20-21 -0.007731507433987872 1101 22-23 -0.2179110140644127 1101 24-25 -0.14668468070927787 1101 26-27 -0.2141791516462419 1101 28-29 -0.33910759307718763 1101 30-31 -0.21380275513212865 1101 32-33 -0.03326854777327526 1101 34-35 -0.35688232366650396 1101 36-37 -0.5069795678020128 1101 38-39 -0.20602703052590954 1101 40-41 -0.4141417842622559 1101 42-43 -0.12170144302937302 1101 44-45 -0.14744758370269295 1101 46-47 0.12702926683427762 1101 48-49 -0.02393251496092219 1101 50-51 -0.25028754014897103 1101 52-53 -0.2669108442073238 1101 54-55 -0.04334728416607092 1101 56-57 0.18004807132870226 1101 58-59 0.18980979633281336 1101 60-61 0.19433741048066722 1101 62-63 0.07151045758896046 1101 64-65 0.18018038774960488 1101 66-67 0.16596908082765083 1101 68-69 0.21970914663945607 1101 70-71 -0.114566358583712 1101 72-73 0.08315514857057948 1101 74-75 0.028887255988841787 1101 76-77 0.08618553460565792 1101 78-79 0.012666944667863334 1101 80-81 -0.002117761176489097 1101 82-83 0.10247748913298338 1101 84-85 0.217743186485297 1101 86-87 0.09389580404524267 1101 88-89 0.20201701569871844 1101 90-91 0.12166811507121622 1101 92-93 -0.10685132954897725 1101 94-95 -0.052375209845752835 1101 96-97 -0.31582645444073165 1101 98-99 -7.788952010372441E-4 1101 100-101 -0.09374389467415867 1101 102-103 -0.14739303566871698 1101 104-105 -0.06617618948878246 1101 106-107 -0.19391586047884246 1101 108-109 -0.15590271505331543 1101 110-111 -0.1719084321711115 1101 112-113 -0.09163522267306234 1101 114-115 -0.16009164691073963 1101 116-117 -0.04511591140777327 1101 118-119 -0.23804843895856465 1101 120-121 -0.02048527283596968 1101 122-123 -0.06725276275571801 1101 124-125 -0.2420893996254776 1102 1 0.07026055672426423 1102 2 0.18457676472694118 1102 3 -0.018329541321620013 1102 4 0.2744231816669753 1102 5 0.30623633236406533 1102 6 0.033142825791451 1102 7 0.09294059148700029 1102 8 0.2904566910315154 1102 9 0.053710871415106 1102 10-11 0.1914290203997311 1102 12-13 0.20742692360137482 1102 14-15 0.2145333232615272 1102 16-17 0.05446388116806844 1102 18-19 0.09098681402991815 1102 20-21 0.15393409218861365 1102 22-23 0.1534708641577751 1102 24-25 -0.0917490898674842 1102 26-27 -0.09798647718452713 1102 28-29 -0.22269391165625052 1102 30-31 -0.12039560084945578 1102 32-33 -0.18465930591634105 1102 34-35 -0.21347011496457213 1102 36-37 0.04585249267658753 1102 38-39 0.2439376876456194 1102 40-41 0.07638365730569063 1102 42-43 0.2585672935045622 1102 44-45 0.10908996072168975 1102 46-47 0.16510242311097656 1102 48-49 0.09797214878145155 1102 50-51 0.1277181049435825 1102 52-53 0.02358694671414696 1102 54-55 0.011891076299253456 1102 56-57 0.03724487059293935 1102 58-59 0.0923100490537756 1102 60-61 0.22992547729414525 1102 62-63 0.21635822046335917 1102 64-65 0.3875706424328733 1102 66-67 0.41760036986313054 1102 68-69 0.37304783831789834 1102 70-71 0.33165065637538405 1102 72-73 0.23063864242302046 1102 74-75 -0.10654243902155258 1102 76-77 0.18081367800845172 1102 78-79 0.024243546869211485 1102 80-81 -0.06431486767012728 1102 82-83 -0.02775705574134335 1102 84-85 -0.11192437147700218 1102 86-87 -0.10535931330647941 1102 88-89 0.19744970371815995 1102 90-91 -0.04966084040826857 1102 92-93 -0.1270432389103533 1102 94-95 0.049463905016420284 1102 96-97 0.0619993127903804 1102 98-99 0.12693835067564763 1102 100-101 0.035317727616028805 1102 102-103 0.04377253323094621 1102 104-105 0.22713076836528856 1102 106-107 0.2586112500385127 1102 108-109 0.46860447907007696 1102 110-111 0.14365134971582805 1102 112-113 -0.015114783989112368 1102 114-115 0.1297758281987207 1102 116-117 -0.036678499983988644 1102 118-119 0.12175683706228213 1102 120-121 0.07363445545431091 1102 122-123 -0.007011518298234876 1102 124-125 0.08534826749244928 1103 1 0.13375589825171375 1103 2 0.10269851444310518 1103 3 0.07413241868537312 1103 4 0.09722336387555686 1103 5 0.04754264138546915 1103 6 0.18431665811015563 1103 7 0.055093775147120994 1103 8 0.0705386453667245 1103 9 -0.08265999616055808 1103 10-11 0.2692060955172906 1103 12-13 0.1604973456355694 1103 14-15 0.18313840988471242 1103 16-17 0.10486767474274217 1103 18-19 0.009610032580404493 1103 20-21 0.07190120176177572 1103 22-23 -0.12920538855089347 1103 24-25 -0.13786446030720612 1103 26-27 0.053867178893817425 1103 28-29 0.16558723104053996 1103 30-31 0.056080916081420185 1103 32-33 0.11920412855724294 1103 34-35 0.09421535192514341 1103 36-37 0.2640902472029012 1103 38-39 0.5335025905582853 1103 40-41 0.15821003150320934 1103 42-43 0.10435451064402201 1103 44-45 -0.020893725484313563 1103 46-47 0.1659912333897502 1103 48-49 0.21316324546167564 1103 50-51 0.1851538255320655 1103 52-53 0.42846152021860817 1103 54-55 0.23771904333965566 1103 56-57 0.2957384680957702 1103 58-59 0.4993164826765941 1103 60-61 0.3705566067007702 1103 62-63 0.38094325623809766 1103 64-65 0.07362694330231534 1103 66-67 0.1170234089717681 1103 68-69 -0.1016969066345581 1103 70-71 -0.07646493737853532 1103 72-73 0.2878866209848603 1103 74-75 0.04606860791691858 1103 76-77 0.19033615122309655 1103 78-79 0.2712285581386702 1103 80-81 0.1508888768940011 1103 82-83 -0.09610997517926023 1103 84-85 0.10243749831500892 1103 86-87 0.16114345786464312 1103 88-89 0.12033318409961424 1103 90-91 0.01992672988382438 1103 92-93 0.09954360116406846 1103 94-95 0.006450724538037633 1103 96-97 -0.0625650515991083 1103 98-99 0.0025903836747680486 1103 100-101 -0.037697122778279635 1103 102-103 -0.17191143567502465 1103 104-105 -0.0519907372862356 1103 106-107 0.1431657135063844 1103 108-109 -0.1255565462780588 1103 110-111 -0.05496736302602656 1103 112-113 -0.25987109977910805 1103 114-115 -0.1063127346558268 1103 116-117 -0.10107500323934104 1103 118-119 0.0950688139166509 1103 120-121 0.25463654334327046 1103 122-123 0.020535177590311093 1103 124-125 0.07662221571715122 1104 1 0.15220210682346647 1104 2 0.07670698728571779 1104 3 -0.030766179303462593 1104 4 0.2999697883399435 1104 5 -0.179049293723601 1104 6 0.21873534682554663 1104 7 0.2747938293685479 1104 8 0.0968171687166901 1104 9 0.17745686789460535 1104 10-11 0.09959780063217494 1104 12-13 0.28520910810997435 1104 14-15 0.14070021337276017 1104 16-17 0.06293296290082395 1104 18-19 -0.0815172169632774 1104 20-21 0.11341237077969168 1104 22-23 -0.05092097035871035 1104 24-25 -0.030016951612907405 1104 26-27 0.03776808278285415 1104 28-29 -0.05116391247543106 1104 30-31 -0.27409032829037727 1104 32-33 -0.35900650480065366 1104 34-35 -0.12216637088618398 1104 36-37 -0.18653051861019065 1104 38-39 -0.12302205437081426 1104 40-41 -0.253829111202414 1104 42-43 -0.12778816916554092 1104 44-45 0.20708055806792913 1104 46-47 -0.030953697105530864 1104 48-49 -0.044085146267271114 1104 50-51 0.08659198430749626 1104 52-53 -0.28813520147055627 1104 54-55 -0.41952209900449944 1104 56-57 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-0.18191031778580324 1104 120-121 -0.06667207984959589 1104 122-123 -0.22794890653432986 1104 124-125 -0.0657835075231823 1105 1 0.13609385567246335 1105 2 0.2551341013791557 1105 3 0.047854006207110444 1105 4 0.19187486895352635 1105 5 0.2454744409449674 1105 6 0.14691220101515512 1105 7 0.23719022948350243 1105 8 0.2708379725958636 1105 9 0.1320657970136736 1105 10-11 0.17204731949666296 1105 12-13 0.32714620656096116 1105 14-15 0.09608788959141634 1105 16-17 0.11956933477836884 1105 18-19 0.09949977370718699 1105 20-21 0.09606391062550301 1105 22-23 0.2604756438514215 1105 24-25 0.06303199517383007 1105 26-27 -0.18333581895709727 1105 28-29 -0.20714623322858472 1105 30-31 -0.008375549358113688 1105 32-33 -0.03154166173627715 1105 34-35 -0.12374170635018089 1105 36-37 0.026325414622967003 1105 38-39 -0.10493052964667982 1105 40-41 -0.1850438023749419 1105 42-43 0.08762967325655069 1105 44-45 0.05430532521531006 1105 46-47 0.020618166544775107 1105 48-49 0.3399037169259138 1105 50-51 0.012430186216608519 1105 52-53 0.26623436538210754 1105 54-55 0.4186884896415144 1105 56-57 0.2633471220675787 1105 58-59 0.0571964293738354 1105 60-61 0.03154324190214908 1105 62-63 -0.0017382120869200435 1105 64-65 -0.09714076713054354 1105 66-67 -0.16728213373291823 1105 68-69 -0.10106639261778483 1105 70-71 0.14904064233665437 1105 72-73 0.08249548767168235 1105 74-75 -0.13887422134541794 1105 76-77 -0.11115065996895623 1105 78-79 -0.21035966946829987 1105 80-81 -0.34646744746682856 1105 82-83 -0.28791930239886554 1105 84-85 -0.31669168647138335 1105 86-87 -0.516420163479733 1105 88-89 -0.27787600347210883 1105 90-91 -0.2746048726042716 1105 92-93 -0.4217913334141379 1105 94-95 -0.22233762974407156 1105 96-97 -0.1782438652788798 1105 98-99 -0.11060023664228424 1105 100-101 -0.39012202626957304 1105 102-103 7.015536768300024E-4 1105 104-105 -0.09750984300560006 1105 106-107 -0.1455343497357937 1105 108-109 -0.13587179374765412 1105 110-111 -0.10797298805046651 1105 112-113 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118-119 0.015665389183691758 2313 120-121 0.3871522354476653 2313 122-123 0.527133908668727 2313 124-125 0.3358659452284769 2314 1 -0.15958282184491068 2314 2 -0.15892563677344285 2314 3 -0.04892776787338704 2314 4 0.010352336876508161 2314 5 -0.09869325255421302 2314 6 0.0011898524397437882 2314 7 0.034100127740238406 2314 8 -0.12661057849747692 2314 9 -0.051297881519296595 2314 10-11 -0.36494061803104927 2314 12-13 -0.10919222108770299 2314 14-15 0.005549870223617859 2314 16-17 -0.026092221225390233 2314 18-19 -0.2610727186247743 2314 20-21 0.02858773211808341 2314 22-23 0.16292604426501356 2314 24-25 0.0730341612284846 2314 26-27 -0.13451172098603337 2314 28-29 0.17197769983624056 2314 30-31 0.171190892324951 2314 32-33 0.30463819436413786 2314 34-35 0.18608176019550626 2314 36-37 0.13682638628239374 2314 38-39 0.08719136610069711 2314 40-41 0.11286074409203906 2314 42-43 -0.13188872696904497 2314 44-45 -0.0295572610948156 2314 46-47 -0.3765121175557731 2314 48-49 -0.39431320323045327 2314 50-51 0.03808414059459153 2314 52-53 -0.08958098050208108 2314 54-55 -0.3075508103550142 2314 56-57 -0.1594151493472431 2314 58-59 -0.036023104352249646 2314 60-61 -0.1460839678312027 2314 62-63 -0.3060310871975247 2314 64-65 -0.0017745915043825278 2314 66-67 -0.03229146207206668 2314 68-69 -1.7052316544140957 2314 70-71 -0.9728244533273518 2314 72-73 -0.16126790968286997 2314 74-75 -0.1292878969153861 2314 76-77 0.15531850746911502 2314 78-79 0.20007856834406468 2314 80-81 -0.07273229056069397 2314 82-83 -0.12965835820338256 2314 84-85 -0.2231697224728464 2314 86-87 0.0029246458563711997 2314 88-89 -0.2502777085249406 2314 90-91 -0.24013241930216012 2314 92-93 -0.17449430397418553 2314 94-95 -0.23381312165303925 2314 96-97 0.025784178998549123 2314 98-99 -0.1324400957261247 2314 100-101 -0.018293201803778913 2314 102-103 0.0457422080705463 2314 104-105 0.15284724949514938 2314 106-107 0.20651594360113634 2314 108-109 0.24054661444738556 2314 110-111 0.21978071110089203 2314 112-113 0.03416095567088462 2314 114-115 0.2852986440399441 2314 116-117 0.06391250830748163 2314 118-119 0.07480374728702088 2314 120-121 0.3338082864030767 2314 122-123 0.21088098254318766 2314 124-125 0.7750297538190338 2315 1 -0.11175589392378882 2315 2 -0.013641081733680949 2315 3 -0.13385795607734963 2315 4 -0.5034930695792852 2315 5 0.004204926581415691 2315 6 -0.2513786099014723 2315 7 -0.1897114523813883 2315 8 -0.16523872272721007 2315 9 0.1224421774968647 2315 10-11 -0.3752459546750444 2315 12-13 -0.06662591787419814 2315 14-15 -0.2024033666686833 2315 16-17 -0.13119788353781558 2315 18-19 -0.14013232854237145 2315 20-21 0.06601036337505661 2315 22-23 0.08620391500523539 2315 24-25 0.15653730096003216 2315 26-27 -0.13771105345571755 2315 28-29 0.028583506090512856 2315 30-31 0.24827962347756483 2315 32-33 0.2873932863069868 2315 34-35 0.22300171953193626 2315 36-37 0.02706510484009783 2315 38-39 -0.19860967028060372 2315 40-41 0.10697937016710313 2315 42-43 0.023210862796688048 2315 44-45 0.1516497812600761 2315 46-47 0.21940825561346955 2315 48-49 0.02665485067576867 2315 50-51 0.1519578856016075 2315 52-53 0.2325226396353841 2315 54-55 0.1731261211646533 2315 56-57 0.22035179980530017 2315 58-59 0.3756786931215679 2315 60-61 0.18730334369493917 2315 62-63 0.3458759173726307 2315 64-65 0.22617981491542238 2315 66-67 0.12675415849722782 2315 68-69 0.604073511298953 2315 70-71 0.42178649884796826 2315 72-73 0.6590409355436613 2315 74-75 0.624109355594598 2315 76-77 0.5519502682377642 2315 78-79 0.6966014730905492 2315 80-81 0.7493690804854047 2315 82-83 0.8190151277083118 2315 84-85 0.652345319450216 2315 86-87 0.6965163098241298 2315 88-89 0.5967829539674128 2315 90-91 0.6528254507183497 2315 92-93 0.47377929349243786 2315 94-95 0.5169988003304802 2315 96-97 0.5808801702180943 2315 98-99 0.26271851019708237 2315 100-101 0.5426706687924963 2315 102-103 0.3135257735101078 2315 104-105 0.49635434762750563 2315 106-107 0.57774759101558 2315 108-109 0.5888375299175372 2315 110-111 0.6398502530059602 2315 112-113 0.897771556987955 2315 114-115 0.9314333556164662 2315 116-117 0.8264197053998572 2315 118-119 0.6914539955867056 2315 120-121 0.7720392410973815 2315 122-123 0.5632776143039564 2315 124-125 0.8866319165452907 2316 1 -0.4924002728819481 2316 2 -0.33839535562905354 2316 3 -0.2752854554367268 2316 4 -0.26334041738467917 2316 5 -0.016713823647378945 2316 6 -0.49082199680339755 2316 7 -0.7323954952850755 2316 8 -0.5847965987750925 2316 9 -0.9455580604502032 2316 10-11 -0.8451502267400599 2316 12-13 -0.892627125913144 2316 14-15 -0.6516176837605983 2316 16-17 -0.5155544655197275 2316 18-19 -0.43314812456682006 2316 20-21 -0.22369705325195355 2316 22-23 -0.35607447391009117 2316 24-25 -0.4389698466037544 2316 26-27 -0.512847186632861 2316 28-29 -0.4776843399394295 2316 30-31 -0.44670770126526804 2316 32-33 -0.25180506087608734 2316 34-35 -0.31534322134736925 2316 36-37 -0.6327014782400298 2316 38-39 -0.7234248037139963 2316 40-41 -0.5060203186713537 2316 42-43 -0.48643743313624555 2316 44-45 -0.4652760341662159 2316 46-47 -0.3245581846984287 2316 48-49 -0.7535472670531185 2316 50-51 -0.8889941772164143 2316 52-53 -0.8390696838261995 2316 54-55 -0.47069572054563835 2316 56-57 -0.4002358836884987 2316 58-59 -0.2781094699270774 2316 60-61 -0.2662146121567268 2316 62-63 -0.2230955564348882 2316 64-65 -0.10275912420673095 2316 66-67 -0.16092270023543165 2316 68-69 -0.13759948536978683 2316 70-71 -0.2180748969861277 2316 72-73 0.05906427265974301 2316 74-75 0.11190427267919745 2316 76-77 0.19434328357619535 2316 78-79 -0.024803695851868923 2316 80-81 0.005551128294463581 2316 82-83 0.13774751047361988 2316 84-85 -0.04648462161212663 2316 86-87 0.1141511617478379 2316 88-89 -0.17198626483406798 2316 90-91 0.003260848089951196 2316 92-93 -0.02844917229809596 2316 94-95 -0.13514318085340093 2316 96-97 -0.16005267379844668 2316 98-99 -0.13701654489681303 2316 100-101 -0.05220157193672037 2316 102-103 0.1577276441401203 2316 104-105 0.24068136926238637 2316 106-107 0.2583070869338755 2316 108-109 -0.019489015823207723 2316 110-111 -0.11160717277286025 2316 112-113 -0.03331988789984308 2316 114-115 0.1372324001674805 2316 116-117 0.21352983828414196 2316 118-119 0.1666583097500478 2316 120-121 0.3197476278162732 2316 122-123 0.04425100083981448 2316 124-125 -0.034170605693603306 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 5.0 15 101.0 16 223.0 17 363.0 18 1279.0 19 6188.0 20 21831.0 21 52329.0 22 77594.0 23 87172.0 24 83843.0 25 56193.0 26 35702.0 27 31111.0 28 23769.0 29 21826.0 30 23811.0 31 24813.0 32 29283.0 33 36868.0 34 46221.0 35 59739.0 36 84750.0 37 173943.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 57.88524186570587 14.194446686164525 9.840322562521006 18.0799888856086 2 14.60605521999434 25.045022406499978 38.79138716000805 21.55753521349763 3 22.79759825282904 37.711271418268886 24.730784081785252 14.760346247116821 4 12.515054287369109 16.9151454047522 33.195840062433795 37.3739602454449 5 12.298292979160475 39.754350803967895 31.52671669950774 16.420639517363888 6 24.387281565993195 29.336732869778753 26.2870585735635 19.988926990664556 7 24.125648396145273 32.87815362110988 25.429795475579038 17.56640250716581 8 28.257625207235865 24.3300778277289 28.58501445926634 18.827282505768896 9 32.19099511010187 13.08065624945733 25.478749321982473 29.24959931845832 10-11 22.978741660767533 25.20248591102571 29.630974598475724 22.18779782973103 12-13 24.828363247823958 24.418130724842868 33.433082351931695 17.32042367540147 14-15 24.01591694017204 21.58235754992303 35.73374520024883 18.667980309656095 16-17 17.75001353481307 25.83949039641169 35.71540935914448 20.69508670963076 18-19 18.041905824259903 23.36966792208442 41.040413419588404 17.54801283406728 20-21 20.24762068201157 23.17292792226829 39.94388926173468 16.635562133985456 22-23 18.70687251067238 26.644118214906293 36.925711670611456 17.72329760380988 24-25 17.029248654937785 28.157895543290728 37.88851535980861 16.924340441962876 26-27 17.865353977170276 27.256423445544314 37.79648783707095 17.081734740214465 28-29 17.080906033155156 27.810232586648553 38.94946533419486 16.159396046001433 30-31 18.705503767079556 27.969403652151705 38.24170604009705 15.08338654067169 32-33 15.613031810292604 29.290671030762173 37.93345980220595 17.162837356739267 34-35 14.504070621802228 30.735531402705778 37.4168266669527 17.34357130853929 36-37 15.935235906275938 31.453029432420355 34.778573708740346 17.833160952563365 38-39 15.572389798530475 30.960399690691215 34.88033693001837 18.58687358075993 40-41 14.758536011085285 31.55516261975136 34.828651557706145 18.85764981145721 42-43 15.84472045248157 30.96862272806671 34.23132987455016 18.955326944901564 44-45 15.794922555331848 30.25500609321962 33.75505767873359 20.19501367271494 46-47 15.328353444423163 29.852774053501886 33.986132924727066 20.83273957734789 48-49 15.016145789172056 29.77659458183887 33.5853867583076 21.621872870681475 50-51 13.895166019316306 29.191551005146792 34.008317822124056 22.90496515341284 52-53 14.526736925773301 28.19671528204531 33.237655489267276 24.038892302914114 54-55 15.047555996234138 27.25426762641967 33.388809794915886 24.309366582430307 56-57 15.835068419256695 25.500195133313852 33.543365569493 25.12137087793645 58-59 15.28261281551335 25.376536988613346 34.492703725858085 24.848146470015216 60-61 16.070778160284803 25.277936280652426 34.144516138260364 24.5067694208024 62-63 14.794400337937061 25.698846794884567 35.022763248425115 24.48398961875326 64-65 14.30928915529732 25.800706472662494 34.63248303280665 25.257521339233545 66-67 14.923689191660102 25.7464832469659 34.173308940024945 25.15651862134905 68-69 14.972056995353217 25.380787920204874 34.40181744448429 25.245337639957626 70-71 14.682105547026069 24.869938107598188 34.047001042946725 26.400955302429015 72-73 15.372636387502208 24.444178855659647 33.7564877721902 26.426696984647947 74-75 15.634752088191819 23.251583062381698 33.62972020221521 27.483944647211267 76-77 15.286984004404689 23.552158062100787 33.04961300649569 28.111244926998836 78-79 15.427184237918521 23.644501239584578 31.806912867470178 29.12140165502673 80-81 15.137386013890294 23.966119043022317 30.777449877778086 30.1190450653093 82-83 15.307107462329805 23.796857267479574 29.31752875764717 31.578506512543452 84-85 15.669318645555027 23.39715482180224 28.65940143285515 32.27412509978758 86-87 16.07938897923494 22.800964705113223 27.92894850820061 33.19069780745124 88-89 16.228238639962246 22.84720618971636 27.849484224354743 33.07507094596665 90-91 16.695203642653038 23.12005859955642 27.273210215692544 32.911527542098 92-93 15.896149962021159 23.78655431536179 26.919259588827327 33.398036133789724 94-95 15.661620320516882 24.223227768049252 27.505685568158366 32.6094663432755 96-97 15.73721400212104 24.380904118042515 27.6567637208485 32.22511815898795 98-99 15.955234921134423 24.603679780619032 28.082541680544853 31.358543617701688 100-101 15.894647360053622 24.77169439642313 28.588111965277523 30.745546278245726 102-103 15.628512111292295 25.78916502172145 28.83895475481121 29.743368112175045 104-105 15.62416518752155 25.878765435251754 29.54307823932781 28.95399113789889 106-107 14.883110634631286 26.877428970257068 30.024817697945274 28.21464269716637 108-109 14.85879243964701 27.35081194219515 30.155043827615124 27.635351790542718 110-111 14.447242212359097 27.693203466962217 30.963323135384158 26.89623118529452 112-113 14.11964799199146 28.155073522276304 31.11409615355149 26.611182332180743 114-115 14.066457498366884 28.001142032357922 31.883847750129856 26.048552719145334 116-117 14.168811382323934 28.098081988321237 32.10311183387618 25.629994795478655 118-119 13.97364746357603 29.11231034662401 32.213416932510825 24.700625257289136 120-121 14.026101248573738 29.97782333646932 32.117856044749665 23.878219370207272 122-123 13.657596809665797 31.440604643513453 31.315931138957076 23.585867407863674 124-125 12.691752165277665 33.214332078411005 31.822707381621434 22.27120837468989 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 22.0 1 15.5 2 52.5 3 131.5 4 162.5 5 189.0 6 284.0 7 433.5 8 667.5 9 917.5 10 1094.5 11 1286.0 12 1632.0 13 2047.5 14 2429.5 15 3002.5 16 3675.5 17 4323.5 18 5172.5 19 6116.0 20 7072.5 21 8115.5 22 9346.0 23 10685.0 24 12079.0 25 13613.5 26 15386.0 27 17565.5 28 20250.0 29 23188.5 30 26070.0 31 28642.5 32 30803.5 33 31926.0 34 31260.0 35 29416.0 36 26974.0 37 24256.0 38 21355.0 39 18622.0 40 16343.0 41 14512.5 42 13641.5 43 13783.5 44 14507.0 45 16057.0 46 19388.0 47 23330.0 48 30802.0 49 40235.5 50 53209.5 51 58620.0 52 45734.0 53 33468.0 54 28900.5 55 25248.0 56 20730.5 57 16135.0 58 12393.5 59 9775.0 60 7482.5 61 5760.5 62 4428.0 63 3231.0 64 2258.0 65 1684.0 66 1308.5 67 1010.5 68 788.5 69 649.5 70 582.0 71 496.0 72 432.0 73 442.0 74 358.0 75 266.0 76 229.5 77 153.0 78 90.0 79 68.0 80 54.5 81 35.5 82 25.0 83 18.0 84 9.5 85 5.5 86 4.5 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004698878500281422 2 0.0 3 3.064485978444406E-4 4 0.0 5 0.0 6 0.0 7 3.064485978444406E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0028601869132147783 24-25 6.128971956888812E-4 26-27 0.010368177560403572 28-29 0.0076101401798036065 30-31 0.00837626167441471 32-33 0.006128971956888811 34-35 0.00536285046227771 36-37 0.001991915885988864 38-39 0.0 40-41 1.532242989222203E-4 42-43 0.0 44-45 0.0 46-47 0.001991915885988864 48-49 0.006537570087348065 50-51 0.009704205598407284 52-53 0.015475654191144248 54-55 0.01670144858252201 56-57 0.01419878503345908 58-59 0.013841261669307232 60-61 0.01843799063697384 62-63 0.007814439245033234 64-65 9.193457935333216E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 2.553738315370338E-4 86-87 5.107476630740676E-4 88-89 0.004392429902436982 90-91 0.011236448587629486 92-93 0.012513317745314657 94-95 0.016650373816214606 96-97 0.01951056072942938 98-99 0.020736355120807144 100-101 0.02298364483833304 102-103 0.020532056055577518 104-105 0.00709939251672954 106-107 0.00188976635337405 108-109 8.171962609185081E-4 110-111 2.0429906522962703E-4 112-113 2.0429906522962703E-4 114-115 5.107476630740676E-5 116-117 5.107476630740676E-5 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.06905308404761394 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 978957.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.06361002951739 #Duplication Level Percentage of deduplicated Percentage of total 1 73.1286426830284 27.104114943916848 2 12.369125368867106 9.168888781558017 3 5.814648992679144 6.4653564816955775 4 2.9132395037657703 4.319006915606371 5 1.5931551341521692 2.9524040304369734 6 0.879980944202032 1.956916232958637 7 0.5632222191502598 1.4612534083381228 8 0.3907634426159396 1.1586483080727106 9 0.27708219689229674 0.9242699842564237 >10 1.7129778375471127 12.409310061094141 >50 0.18324451925707194 4.756934288925245 >100 0.14319362207310568 10.803248158930037 >500 0.019476527139669717 5.045569126707096 >1k 0.01014973949532084 7.748211260389602 >5k 8.229518509719598E-4 2.2894475798007172 >10k+ 2.7431728365732E-4 1.4364204373134617 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14128 1.4431685967820855 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8880 0.907087849619544 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7760 0.7926803730909529 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 5878 0.6004349527098739 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 4582 0.46804915844107553 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4494 0.45905999957097193 No Hit GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4436 0.4531353266793128 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3565 0.3641630837718102 No Hit CTGCACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 3351 0.3423030837922401 TruSeq Adapter, Index 11 (95% over 21bp) GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 3234 0.3303515884763069 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3131 0.31983018661698115 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 2967 0.3030776632681517 No Hit GCACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT 2914 0.29766373803956664 TruSeq Adapter, Index 11 (95% over 23bp) GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 2848 0.2909218688869889 No Hit GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA 2781 0.28407785020179643 No Hit GTACATGGGCCATCTCATCCCTGCACATCTCCGAGCCCACGAGACCGTAC 2246 0.22942785025287116 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1984 0.20266467270779 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 1902 0.19428841103337532 No Hit GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1857 0.1896916820657087 No Hit GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA 1850 0.188976635337405 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1792 0.18305196244574581 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1676 0.17120261666242748 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1674 0.17099831759719783 No Hit GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 1634 0.1669123362926053 No Hit GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1572 0.16057906527048688 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1494 0.1526114017265314 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA 1427 0.1457673830413389 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 1413 0.1443372895847315 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA 1379 0.14086420547582784 No Hit GAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAA 1369 0.13984271014967972 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1298 0.13259009333402794 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 1274 0.1301385045512724 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA 1232 0.12584822418145028 No Hit GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA 1200 0.12257943913777623 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1162 0.11869775689841332 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 1116 0.11399887839813189 No Hit GTGTTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1110 0.113385981202443 No Hit GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1093 0.11164943914799119 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1073 0.10960644849569491 No Hit CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC 1053 0.10756345784339864 No Hit GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC 1025 0.10470327093018386 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 998 0.10194523354958389 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.0214953261481351E-4 0.0 2 0.0 0.0 0.0 1.0214953261481351E-4 0.0 3 1.0214953261481351E-4 0.0 0.0 1.0214953261481351E-4 0.0 4 1.0214953261481351E-4 0.0 0.0 2.0429906522962703E-4 0.0 5 2.0429906522962703E-4 0.0 0.0 2.0429906522962703E-4 0.0 6 2.0429906522962703E-4 0.0 0.0 2.0429906522962703E-4 0.0 7 2.0429906522962703E-4 0.0 0.0 2.0429906522962703E-4 0.0 8 2.0429906522962703E-4 0.0 0.0 2.0429906522962703E-4 0.0 9 2.0429906522962703E-4 0.0 0.0 4.0859813045925406E-4 0.0 10-11 2.0429906522962703E-4 0.0 0.0 0.002553738315370338 0.0 12-13 2.0429906522962703E-4 0.0 0.0 0.007661214946111014 0.0 14-15 2.0429906522962703E-4 0.0 0.0 0.010061728962559132 0.0 16-17 2.0429906522962703E-4 0.0 0.0 0.016905747647751636 0.0 18-19 3.064485978444406E-4 0.0 0.0 0.026303504648314484 0.0 20-21 3.064485978444406E-4 0.0 0.0 0.05041079434541047 0.0 22-23 3.064485978444406E-4 0.0 0.0 0.13882121482353157 0.0 24-25 3.064485978444406E-4 0.0 0.0 0.33096448567199577 0.0 26-27 3.064485978444406E-4 0.0 0.0 0.5846528499208852 0.0 28-29 3.5752336415184735E-4 0.0 0.0 0.9762430831997728 0.0 30-31 4.0859813045925406E-4 0.0 0.0 1.4342815874445967 0.0 32-33 4.0859813045925406E-4 0.0 0.0 2.1036163999031623 0.0 34-35 4.0859813045925406E-4 0.0 0.0 2.762685184333939 0.0 36-37 4.0859813045925406E-4 0.0 0.0 3.5856018190788768 0.0 38-39 4.0859813045925406E-4 0.0 0.0 4.4647006967619625 0.0 40-41 4.0859813045925406E-4 0.0 0.0 5.776913592731857 0.0 42-43 4.0859813045925406E-4 0.0 0.0 6.792586395520948 0.0 44-45 4.0859813045925406E-4 0.0 0.0 7.598341908786596 0.0 46-47 4.0859813045925406E-4 0.0 0.0 8.338108823983076 0.0 48-49 4.0859813045925406E-4 0.0 0.0 8.964438683210805 0.0 50-51 4.0859813045925406E-4 0.0 0.0 9.540664196690969 0.0 52-53 4.0859813045925406E-4 0.0 0.0 10.204789382986178 0.0 54-55 4.0859813045925406E-4 0.0 0.0 10.80685872821789 0.0 56-57 4.0859813045925406E-4 0.0 0.0 11.34728083051656 0.0 58-59 4.0859813045925406E-4 0.0 0.0 11.922076250540115 0.0 60-61 4.0859813045925406E-4 0.0 0.0 12.391810876269336 0.0 62-63 4.0859813045925406E-4 0.0 0.0 12.854701483313363 0.0 64-65 4.0859813045925406E-4 0.0 0.0 13.32785811838518 0.0 66-67 4.0859813045925406E-4 0.0 0.0 13.811127557185863 0.0 68-69 4.0859813045925406E-4 0.0 0.0 14.307982883824316 0.0 70-71 4.0859813045925406E-4 0.0 0.0 14.867098350591498 0.0 72-73 4.0859813045925406E-4 0.0 0.0 15.354249471631544 1.0214953261481351E-4 74-75 4.0859813045925406E-4 0.0 0.0 15.813820218865589 1.0214953261481351E-4 76-77 4.0859813045925406E-4 0.0 0.0 16.25832391003895 1.0214953261481351E-4 78-79 4.0859813045925406E-4 0.0 0.0 16.69475778813574 1.0214953261481351E-4 80-81 4.0859813045925406E-4 0.0 0.0 17.12986372230854 1.0214953261481351E-4 82-83 4.0859813045925406E-4 0.0 0.0 17.705987086256087 1.0214953261481351E-4 84-85 4.0859813045925406E-4 0.0 0.0 18.106821852236614 1.0214953261481351E-4 86-87 4.0859813045925406E-4 0.0 0.0 18.49764596402089 1.0214953261481351E-4 88-89 4.0859813045925406E-4 0.0 0.0 18.87222830011941 1.0214953261481351E-4 90-91 4.0859813045925406E-4 0.0 0.0 19.240273066130584 1.0214953261481351E-4 92-93 4.0859813045925406E-4 0.0 0.0 19.575221383574558 1.0214953261481351E-4 94-95 4.0859813045925406E-4 0.0 0.0 19.917626616899415 1.0214953261481351E-4 96-97 4.0859813045925406E-4 0.0 0.0 20.25977647639273 1.0214953261481351E-4 98-99 4.0859813045925406E-4 0.0 0.0 20.63456311155648 1.0214953261481351E-4 100-101 4.0859813045925406E-4 0.0 0.0 21.01982007381325 1.0214953261481351E-4 102-103 4.0859813045925406E-4 0.0 0.0 21.372082737035434 1.0214953261481351E-4 104-105 4.0859813045925406E-4 0.0 0.0 21.727205587170836 1.0214953261481351E-4 106-107 4.0859813045925406E-4 0.0 0.0 22.05582063359269 1.0214953261481351E-4 108-109 4.0859813045925406E-4 0.0 0.0 22.382341614595944 1.0214953261481351E-4 110-111 4.0859813045925406E-4 0.0 0.0 22.698749791870327 1.0214953261481351E-4 112-113 4.0859813045925406E-4 0.0 0.0 23.03191049249354 1.0214953261481351E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAAC 370 0.0 78.79253 1 CGCATAG 485 0.0 74.83052 1 AGATTAC 820 0.0 67.47762 2 TATCACG 305 0.0 66.32385 2 TCACGCA 325 0.0 64.073044 4 GAGTTCT 555 0.0 60.032402 1 ATAACGC 510 0.0 59.4964 3 ATAGTAC 620 0.0 58.536785 4 TACAACG 215 0.0 58.112766 2 GCATAGT 625 0.0 58.06849 2 TAGTACT 655 0.0 57.225544 5 GATTACT 970 0.0 56.429573 3 GTATCAC 415 0.0 55.912277 1 ATCACGC 360 0.0 54.538364 3 TAACGCA 605 0.0 52.120815 4 TACGCAG 230 0.0 51.736004 5 CATAGTA 705 0.0 51.479153 3 AGTGTAC 430 0.0 51.19458 3 GTACAAC 260 0.0 50.34311 1 GTGTACT 450 0.0 48.919266 4 >>END_MODULE