FastQCFastQC Report
Fri 10 Feb 2017
SRR3556538.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556538.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5795966
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT946771.633498195123988No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT447250.7716573906748245No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390610.6739342501319021No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155650.26854884932037215No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT106260.18333440879397844No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA95430.16464899897618446No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC90140.15552196130895177No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83780.14454881205307277No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC81730.14101186928977844No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT77540.13378270334919148No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA68980.119013810640021No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA66270.11433814484073923No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC65730.1134064623567495No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA651700.058.6343151
TATCAAC830750.045.8323142
AACGCAG853650.044.6794056
ATCAACG855350.044.465533
TCAACGC877250.043.463884
GGTATCA329950.043.1475751
CAACGCA895350.042.5918735
GACCGTT14350.040.590977
ACGCAGA996150.038.2641147
CGCAGAG1000650.038.1217428
GTACTGG54950.037.978291
GCAGAGT1048750.036.5150039
TACTGGT51200.036.335372
GGACCGT22650.034.113826
GGTTCAC53700.033.8696
TTAGGAC19950.033.0700843
ACTGGTT58550.030.9619393
AGAGTAC1033700.029.60913710-11
TCACTAT62700.029.2918059
TTCACTA61750.029.1649258
Produced by FastQC (version 0.11.5)