FastQCFastQC Report
Fri 10 Feb 2017
SRR3556413.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556413.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11119
Sequences flagged as poor quality0
Sequence length125
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1651.4839463980573793No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT750.6745210900260814No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT690.6205594028239949No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.27880205054411367No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT270.2428275924093893No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.21584674880834606No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA240.21584674880834606No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA210.18886590520730281No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT200.17987229067362173No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT190.17087867613994062No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC180.16188506160625954No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC160.14389783253889737No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT150.1349042180052163No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.1349042180052163No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT140.1259106034715352No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.1259106034715352No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT130.11691698893785411No Hit
ACGTAACACAGTTCCACCCGCCTCACATTGAAATCCAAATGCTGAAGAAC130.11691698893785411No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.11691698893785411No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA130.11691698893785411No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA130.11691698893785411No Hit
TTCTATATCCTGGCTCACACTGAATTCACCCCCACTGAGACTGATACATA130.11691698893785411No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC130.11691698893785411No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG120.10792337440417303No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT120.10792337440417303No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC120.10792337440417303No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1059.276846E-1150.986491
GTATCAA1700.045.487941
AACGCAG1750.044.188296
CAACGCA1750.044.188295
ATCAACG1800.042.9608353
TCAACGC1850.041.7997364
TATCAAC1850.041.7997362
CGCAGAG2150.035.9672138
ACGCAGA2200.035.1497767
GCAGAGT2300.033.6215259
CAGAGTA2150.030.43379410-11
GTACTTT1651.521039E-828.840841
GAGTACT1203.8198777E-1027.26360712-13
AGAGTAC2150.026.28373110-11
AGTACTT1256.1299943E-1026.17306112-13
TACTTTT1855.651964E-825.7229142
GAGTACA1351.4988473E-924.23431812-13
ACTTTTT2001.3767931E-723.7936943
ACATGGG1402.2828317E-923.36880716-17
GTACATG1557.403287E-921.10730714-15
Produced by FastQC (version 0.11.5)