FastQCFastQC Report
Fri 10 Feb 2017
SRR3556397.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556397.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771015
Sequences flagged as poor quality0
Sequence length125
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174202.259359415834971No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88951.1536740530339877No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75860.9838978489393851No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38140.4946726068883226No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA35350.45848654046938125No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA30000.38909748837571256No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA27300.3540787144218984No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20420.2648456904210683No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT19950.25874982976984884No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA17810.23099420893238132No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG16520.2142630169322257No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15820.2051840755367924No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA15720.20388708390887336No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14820.192214159257602No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA14220.18443220949008773No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA13640.17690965804815728No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13470.17470477228069492No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT12160.15771418195495548No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC12150.15758448279216358No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11870.15395290623399024No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11630.15084012632698454No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT11590.15032132967581693No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC11540.14967283386185742No Hit
GAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAA11430.14824614307114647No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA11320.14681945228043553No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG9520.12347360297789278No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC9230.11971232725692756No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9170.11893413228017613No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAACACAGGTGGAAAA9010.11685894567550566No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA8810.11426496241966758No Hit
GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8700.11283827162895663No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC8410.10907699590799141No Hit
GTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8380.1086878984196157No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT8230.10674241097773714No Hit
GTACATGGGACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA8210.10648301265215333No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7900.10246233860560429No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAGT303.570858E-799.196021
GTATAAC3200.081.836721
TACAACG2150.080.253542
CGCATAG5250.078.223151
ACGCATA1450.073.883941
GTACAAC2350.073.4472661
ACAACGC2450.070.4265753
ATAACGC3850.069.543493
GGTTTCA5200.061.806751
ATAGTAC6700.061.2743844
TCACGCA2150.060.8819964
GCATAGT6900.059.4983142
TAGTACT6950.058.2141765
TATCACG2150.058.1146352
TAACGCA5000.055.9284134
AGATTAC7200.055.366492
ATCACGC2550.053.6651463
CATAGTA7800.053.3959243
CGCAAAG2500.052.37551
GAGTTCT4850.051.5410271
Produced by FastQC (version 0.11.5)