FastQCFastQC Report
Fri 10 Feb 2017
SRR3556375.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556375.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98382
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT55885.679900794860848No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT22412.2778557053119473RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT14171.4403041206724807RNA PCR Primer, Index 26 (100% over 24bp)
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC4130.41979223841759666RNA PCR Primer, Index 26 (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT2730.2774897847167165RNA PCR Primer, Index 26 (100% over 21bp)
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG2330.23683194080217929RNA PCR Primer, Index 26 (96% over 27bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT1620.16466426785387572RNA PCR Primer, Index 26 (100% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1270.12908865442865566No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG1210.12298997784147506RNA PCR Primer, Index 26 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGATT250.002054652271.37012
ATACACC250.002054652271.37014
CATGCTA350.007788079350.9786459
GTGTAAG350.007788079350.9786451
CCTATAT350.007788079350.9786453
CGGAGAT350.007788079350.9786454
CTTATTC602.1840528E-549.5625723
TATCAAC1550.046.0452272
CATACAC558.5078593E-443.254613
GTATCAA1700.041.9824141
TAATACC600.001305374439.650064
GCAGAGT2150.035.961689
CGCAGAG2200.035.1443678
TTAATAC851.7033458E-434.9853483
CAACGCA2250.034.3633845
CCGGCCT700.002782371133.9857649
TACATGG1407.967901E-833.9857642
CTGGGCC902.3830465E-433.0417184
AACGCAG2400.032.215676
ATCAACG2255.456968E-1231.7200493
Produced by FastQC (version 0.11.5)