FastQCFastQC Report
Fri 10 Feb 2017
SRR3556289.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556289.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1474707
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162831.1041515365425132No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90840.6159867688971437No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75500.511966105809493No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35400.24004768404842455No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT34300.23258857522206106No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA29890.2026843298363675No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC26820.18186663520278942No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25880.17549248766026065No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA25370.17203417356803757No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT22930.1554885139895586No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC22460.15230144021829423No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT21820.14796159508295545No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21090.14301145922545971No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA17920.12151566378948496No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA17810.1207697529068486No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16050.10883517878466704No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT14840.10063015907566723No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG5150.064.7375261
ACAACGC3650.045.6384773
TACAACG3850.044.8129162
ATAGTAC8050.044.342624
TAACGCA4350.043.7649774
GCATAGT8350.041.3244862
ATAACGC5500.038.9408843
GTACTGG13150.038.9357031
TACTGGT12250.038.366932
TAGTACT10200.036.7444425
AGATTAC7350.036.4242942
GGTTCAC13450.034.500366
GATTACT7800.034.322893
CATAGTA10750.034.3122523
ACTGGTT13800.033.6264883
GTATAAC5250.031.7522161
CATGGGG46700.031.3389324
TCAAACG2109.276846E-1131.1630083
ACATGGG188050.031.1622543
AACGCAG203550.030.9218736
Produced by FastQC (version 0.11.5)