FastQCFastQC Report
Fri 10 Feb 2017
SRR3556282.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556282.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences839547
Sequences flagged as poor quality0
Sequence length125
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT537026.396544803328462No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271783.237221978042921No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258003.073085842722325No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110861.320474017535647No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA84711.0089965183604968No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA83460.9941075365643615No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55650.6628574695639434No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA54390.647849375913439No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA51330.6114011484764998No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42880.5107516315346252No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA39370.46894337065107733No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37280.44404899308793905No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34150.4067669826704163No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA29900.3561444445635563No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAA25220.3004000967188258No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA24930.2969458529421224No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC24560.2925387143304663No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24450.2912284839324064No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20510.24429841331098795No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA20220.24084416953428456No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA17780.21178087706822846No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17690.21070887037890673No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16630.198083013815784No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA16270.19379498705849701No Hit
GTACATGGGACTGCTGCTAGCTGGAGCCACCCGCAGTTCGAAAAAAAAAA15820.18843495361188833No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA15500.1846233742720777No Hit
GTACATGGGACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA14980.1784295578448854No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC14650.1744988666507057No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA13700.16318324048564284No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA13590.16187301008758295No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG12320.14674580458270947No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC12300.1465075808739713No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT11090.13209504649531234No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA11070.13185682278657418No Hit
GTATCAACGCAGAGTACATGGGACTGCTGCTAGCTGGAGCCACCCGCAGT10670.12709234861181087No Hit
GTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAA10660.12697323675744182No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT10570.12590123006812007No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA10390.12375721668947659No Hit
TATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA9840.11720606469917706No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC9820.11696784099043889No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC9780.11649139357296255No Hit
ACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA9470.11279892608752101No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA9380.11172691939819927No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAAAAAAAAA9370.11160780754383018No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA9250.11017846529140118No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAA9070.1080344519127577No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8740.104103760718578No Hit
GTATCAACGCAGAGTACATGGGACAGTGGTATCAACGCAAAAAAAAAAAA8580.10219797104867269No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAA8540.10172152363119634No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCAGA304.2525106E-579.374781
GACATGT304.2525106E-579.374781
GCCATTA201.772081E-459.50094102-103
ATTGTAG551.3063553E-554.1191671
GTAGTAT3400.052.4992643
GCTATTG350.007801465750.9901738
CTATTGA350.007801465750.9901739
CAGTAGT3550.050.3079571
AGTAGTA3550.050.2809872
GCACGTG602.1946043E-549.5767337
TAGTATC3650.048.9034234
AGGGCCG255.3258514E-447.6135196-97
GGCCGGC1250.047.60358498-99
TAGGACC751.470482E-647.5993354
CTTAGGA751.470482E-647.5993352
GTAGCGA505.336846E-447.5908249
TGAGCGG900.046.29091392-93
TGGGCCG909.730138E-846.2716146
TAGTGGT2450.046.136715
ACGTGTG653.5233854E-545.760419
Produced by FastQC (version 0.11.5)