FastQCFastQC Report
Fri 10 Feb 2017
SRR3556277.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556277.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1163720
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185531.5942838483484No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104420.8972948819303612No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85010.7305021826556216No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT42690.36684082081600383No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC38910.3343587804626542No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37720.3241329529440071No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA35330.3035953665830269No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC33740.28993228611693533No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT30290.26028597944522736No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA29940.25727838311621354No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT27670.23777197263946653No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT27160.2333894751314749No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA25670.22058570790224452No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24970.21457051524421683No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA24680.21207850685731963No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT20860.17925274120922557No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT19940.17134705943010345No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC19850.17057367751692848No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19340.16619118000893685No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC18790.1614649572062008No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA18580.15966039940879248No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA17310.1487471213006565No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT16960.14573952497164266No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA16390.14084143952153438No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT15830.13602928539511222No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG15740.13525590348193722No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15720.13508404083456502No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC15230.13087340597394562No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA14530.12485821331591793No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT14500.12460041934485959No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG14100.12116316639741519No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC13800.1185852266868319No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13750.11815557006840133No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA13630.11712439418416802No Hit
GTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC13530.11626508094730692No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG13390.11506204241570138No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA13310.11437459182621248No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA13290.11420272917884028No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG13260.11394493520778194No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGT13200.11342934726566527No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG13200.11342934726566527No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC12810.11007802564190698No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGT12790.10990616299453478No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12740.10947650637610422No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG12620.1084453304918709No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG12360.10621111607603204No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA12230.1050940088681126No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA12160.10449248960230983No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG12150.10440655827862373No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCC12150.10440655827862373No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC11960.10277386312858763No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCG11850.10182861856804042No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG5050.067.211041
ATAGTAC5400.063.9107554
GCATAGT5650.060.029692
TCACGCA2500.057.1229934
TAGTACT6250.055.2094045
TTCAACG7100.053.6366124
AGATTAC5350.053.385972
TACAACG2750.051.9299932
GGTTTCA6800.051.6654471
GTACAAC3000.051.607071
AGCGCGA350.00779996250.9939045
GTATAAC2400.049.6221771
GAGTTCT6300.049.149591
ATAACGC2950.048.4093133
AGTGTAC4750.047.602493
TATCACG2950.046.3922582
ACAACGC3100.046.066933
TACTGGT12400.045.5870632
CATAGTA7650.045.1134723
TAACGCA3350.044.405314
Produced by FastQC (version 0.11.5)