FastQCFastQC Report
Mon 27 Feb 2023
SRR3129005.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3129005.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9737556
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3751393.8524964580434764No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT349370.35878612662150544No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCT202160.20760856214844878No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTT191830.19700015075651425No Hit
CGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCT179960.18481023369724395No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT163430.1678347215666847No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT134440.13806339085495375No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109680.11263606596973615No Hit
ATGATGGTTACAATGAAGGAGGAAATTTTGGCGGTGGTAACTATGGTGGT97810.10044614891046584No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1009750.089.231771
ATAGGGC46750.071.8062743
AGGGACT208350.071.578975
AGTAGGG145000.070.205182
GGATAGG56300.068.782621
ATAGGGA68850.068.629043
GACTTAA158500.068.341858
ATAGAGG64550.067.4997251
TAGGGAT47100.067.4798054
GGTAAGG50200.067.392641
AAGGGGC326950.066.623734
GTAGGGA71650.066.209573
GGACTTA165650.066.186347
TATAGGG70050.065.981872
AGATAGG46650.065.662381
ACTTAAT164400.065.517589
GTTTTTT1382800.065.445982
TAGGGAC46150.064.997554
GAATAGG58400.064.214451
AATAGGG127900.064.1878742