FastQCFastQC Report
Mon 27 Feb 2023
SRR3129003.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3129003.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7299445
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2460713.371091911782334No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT233640.3200791293036662No Hit
CGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCT219200.30029680338710685No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT211980.2904056404288271No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCT207670.2845010819315715No Hit
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG164550.22542809761564064No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC144140.19746706770172254No Hit
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC108900.14918942467543767TruSeq Adapter, Index 22 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTC99900.13685972015680645No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTC99890.13684602048511907No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT98360.13474997071695177No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT78300.10726842931209153No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT722550.089.844981
ATAGGGA45750.072.626223
ATAGGGC33400.071.7610553
GGATAGG38000.069.551981
GGTAAGG39550.069.204331
AGGGACT134000.068.8458255
AGATAGG38450.068.248731
ATAGAGG46100.068.144741
TAGGGAT28800.068.046954
AGTAGGG105000.067.721982
GACTTAA97100.067.419898
AGTAAGG42700.066.632221
GTTTTTT977500.066.590612
GTAGGGA49200.066.578313
AAGGGGC246550.066.277294
ATAAGGG84550.065.980722
GATAGGG97050.065.95712
AATAGGG87450.065.888662
AGGGAAT57950.065.7702565
TATAGGG47800.065.6793062
Produced by FastQC (version 0.11.5)