FastQCFastQC Report
Mon 27 Feb 2023
SRR3129001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3129001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12822255
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4705713.669955089802847No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT463430.36142628578202507No Hit
CGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCT227440.17737909595465073No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCT222720.17369799617929918No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT221020.17237217634495647No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT203450.15866943841001446No Hit
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG190230.14835923946294938No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT164280.12812098963871799No Hit
ATGATGGTTACAATGAAGGAGGAAATTTTGGCGGTGGTAACTATGGTGGT143120.11161843217125224No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130130.10148760884883354No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1339700.089.107641
ATAGGGC61950.071.4914253
ATAGGGA93400.070.725143
AGGGACT262250.070.044775
GGTAAGG72600.068.443051
GACTTAA191700.068.2485358
AGTAGGG190150.068.221852
ATAGAGG88850.067.311641
AGATAGG64200.066.7706151
GTAGGGA92300.066.627023
TAGTAGG48250.066.510331
GTTTTTT1816850.065.9528662
GGATAGG68050.065.897181
ACTTAAT198550.065.6572659
GGACTTA203800.065.487797
AAGGGGC442800.065.460354
TATAGGG93500.065.1720662
AATAGGG172300.065.165412
TAGGGAT63400.065.111564
TAGAGGG234250.064.79212