FastQCFastQC Report
Mon 27 Feb 2023
SRR3129000.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3129000.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2253140
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223190.9905731556849552No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC27010.11987714922286233Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG26060.11566081113468314No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT87450.083.450761
CGTAGGG5950.074.498282
TAGGGCA12750.074.3397754
ACGGGTA2150.072.378714
GAATAGG15900.072.0956341
GTAGGGC11000.072.0198063
AATAGGG36100.071.974682
ACGGGAT5950.070.535614
ATAGCGG5600.069.918141
ATAGGGC14400.069.423853
ATAGGGA20600.068.902443
TAGGGTA5350.068.750554
AGGGATG35250.068.655825
AGTAGGG40300.068.217982
GGTAAGG12050.068.118021
GAGGGAT27700.067.924824
TAGAGGG40550.067.681112
AAGAGGG84000.066.9724962
ATAGAGG17200.066.920811
GTACGGG5200.066.1995242
Produced by FastQC (version 0.11.5)