FastQCFastQC Report
Mon 27 Feb 2023
SRR3128999.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128999.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2294514
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214960.9368432705139302No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC28190.12285826105223153Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG26170.11405465383954948No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81200.083.403711
ATAGGGA20250.074.035523
ATAGGGC15200.071.732993
AGATAGG13700.070.7664261
GAATAGG15600.070.5946661
CGTAGGG8000.069.965152
GGTAAGG13100.069.337251
ATAGAGG17400.069.242271
ATAGCGG4750.068.365361
ACGGGAT5650.068.2087254
GGATAGG15500.068.013791
AGTAGGG39450.067.840662
GATAGGG35150.067.709562
AGGGATG35400.067.44275
AGGGTAC12200.067.4144065
TAGGGCA12300.067.248414
TAGAGGG42800.066.706742
ATAACGG2900.066.53751
AATAGGG37050.066.0145952
AAGAGGG78600.065.94532
Produced by FastQC (version 0.11.5)