FastQCFastQC Report
Mon 27 Feb 2023
SRR3128998.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128998.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2395822
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219800.9174304268013233No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC30250.1262614668368518Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG28400.11853969117906088No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86400.082.804581
GAATAGG16600.075.655771
CGTAGGG7400.074.9375152
GTAGGGT8300.071.910573
ATAGGGA21950.071.512023
ATAGGGC15850.071.162153
TAGGGCA12750.070.0342564
AGTAGGG40000.069.552172
AGGGATG38550.069.364445
ATAGAGG17750.068.8991551
TAGAGGG44750.068.575522
GGATAGG17650.068.490021
AATAGGG37950.068.3559342
ACGGGAT7600.067.403124
GATAGGG38400.066.575842
TAAGGGA19600.066.418763
ATAGCGG5100.066.405071
ATAAGGG33550.065.834622
CGAAGGG27200.065.827152
AAGGGGC74750.065.449554
Produced by FastQC (version 0.11.5)