FastQCFastQC Report
Mon 27 Feb 2023
SRR3128997.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128997.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1945884
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133860.6879135652484938No Hit
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC50240.2581859966986727Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG49040.25201913372020124TruSeq Adapter, Index 23 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG45770.2352144321038664TruSeq Adapter, Index 23 (95% over 23bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26130.1342834413562165No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA2200.083.5971154
CGTTTTT50900.078.780461
AGGGTAC12700.077.7042165
CGTAGGG5750.075.4517442
TAGGGTA9150.075.2456054
AAGGGTA16800.073.82374
CGGGTAT2650.072.705455
AGGGTAT13550.072.136175
ATAGCGG4650.072.0315551
GAGGGTA15050.071.127694
ATAGAGG12300.070.955161
ATAGGGC9600.070.7360153
TAGGGCA8050.070.296674
AGGGTAG11900.069.10675
AGTAAGG13300.068.8125151
AGGGCAT13700.068.6022645
AGTAGGG32000.068.525522
GTAGGGT11250.068.325763
GGTAAGG9050.068.2873151
TATAGGG15250.067.411812
Produced by FastQC (version 0.11.5)