FastQCFastQC Report
Mon 27 Feb 2023
SRR3128996.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128996.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2032170
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136070.6695798087758406No Hit
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC54790.26961327054331086Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG52000.25588410418419716TruSeq Adapter, Index 23 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG49590.24402486012489114TruSeq Adapter, Index 23 (95% over 23bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26790.13182952213643542No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT46350.078.701031
ACGGGTA3500.077.881814
CGTAGGG6050.077.727962
AGGGTAC12950.074.760745
AGTACGG1450.074.660051
AAGGGTA18000.072.3241654
TAGGGTA9300.071.759854
AGGGTAT14050.070.24565
GTAGGGT10750.069.953
TACACGG1750.069.930041
CGGGTAT3300.069.7843865
GAGGGTA14300.069.017534
TAAGGGT11600.068.875863
AGAGGGT28250.068.3753363
AGGGAAT20700.068.339585
AAGAGGG67550.068.293022
TAGGGTC6700.068.0413744
ATAGGGT8400.067.6993
ATAGGGC10400.067.3331763
GGTAAGG10650.067.1772841
Produced by FastQC (version 0.11.5)