FastQCFastQC Report
Mon 27 Feb 2023
SRR3128995.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128995.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2083300
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138350.6640906254500072No Hit
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC55860.26813229011664186Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG54160.2599721595545529TruSeq Adapter, Index 23 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG50960.24461191379062064TruSeq Adapter, Index 23 (95% over 23bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25320.12153794460711372No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA2900.081.025174
CGTTTTT51600.079.318841
CGTAGGG5900.075.669272
AGGGTAG13300.074.908685
GAGGGTA16150.074.2024
AGGGTAT13100.073.899915
AAGGGTA19950.073.730874
AGGGTAC15400.072.628015
ATAGAGG12100.070.3720251
TAGGGTA9400.069.991964
AGAGGGT28100.069.070353
ATGCGGG9050.069.063892
ATAGGGC8650.068.9978563
AAGAGGG70950.068.554492
GGTAAGG9350.068.428051
ATAGCGG4550.068.240051
AGGGATG23800.067.9249655
AAGGGTC17050.067.804534
ATAGGGA15900.067.388493
TAGAGGG35750.067.304152
Produced by FastQC (version 0.11.5)