FastQCFastQC Report
Mon 27 Feb 2023
SRR3128994.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128994.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115660
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC14181.2260072626664362No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG12151.0504928237938786No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG7480.6467231540722808No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5530.478125540376967No Hit
CGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT3830.33114300536053953No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA3020.2611101504409476No Hit
ACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG2940.254193325263704No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCTACAACGTTCGT2400.20750475531730936No Hit
AAAAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA2390.2066401521701539No Hit
AGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT2350.20318173958153207No Hit
AAAACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT2330.20145253328722115No Hit
ACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT2190.1893480892270448No Hit
AGAGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT2030.1755144388725575No Hit
AAAGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT1950.16859761369531384No Hit
GGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT1890.1634099948123811No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT1860.16081618537091474No Hit
GCCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT1800.155628566487982No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG1790.15476396334082657No Hit
GCAGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT1770.15303475704651565No Hit
GAAGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT1630.14093031298633926No Hit
CGTTCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT1570.13574269410340656No Hit
GGAACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT1530.13228428151478472No Hit
GCAGAGAATATCCTGTCTCTTATACACATCTGACGCTACAACGTTCGTAT1500.12969047207331835No Hit
ACACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT1420.1227736468960747No Hit
GGAAGGGATCACATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGC1400.1210444406017638No Hit
AGGCCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT1300.11239840913020924No Hit
GCAGAGACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGT1260.10893999654158742No Hit
GCAGAGAATATCAAAGCGGAAAAAATCCAAAAAAAAAAAAAAAAAAAAAA1230.10634618710012106No Hit
AAGACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT1210.10461698080581014No Hit
AGGGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT1200.10375237765865468No Hit
AGACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT1190.10288777451149922No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTGG201.8044228E-594.27581
ATAGCGG201.8044228E-594.27581
GTAATGG201.8044228E-594.27581
ACGATGG156.8066164E-494.27581
TATAAGG301.288754E-894.27581
AGAATGG156.8066164E-494.27581
TCGAAGG201.8044228E-594.27581
CCGGGCG156.830126E-494.194114
TAGCGGG254.833273E-794.194112
GCACGGG201.8122126E-594.194112
ACGGGCC156.830126E-494.194114
ACGGGAT156.830126E-494.194114
CGTAGGG301.29657565E-894.194112
TGGGGTA156.830126E-494.194114
GCAGGGT201.8122126E-594.194113
GCGTGGG156.830126E-494.194112
GTACGGG156.830126E-494.194112
CAGGGAC353.4924597E-1094.19414
GACCATC201.8318044E-593.990498
CGGGGAT201.8318044E-593.990495
Produced by FastQC (version 0.11.5)