FastQCFastQC Report
Mon 27 Feb 2023
SRR3128993.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128993.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121619
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC14541.1955368815727805No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG14151.1634695236763992No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG7890.648747317442176No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4960.40783101324628557No Hit
CGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT4280.3519186969141335No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTACAACGT3690.303406540096531No Hit
ACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG3460.2844950213371266No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA2890.23762734441164618No Hit
AAAAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA2790.22940494495103564No Hit
AGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT2640.21707134576011972No Hit
AAAACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT2570.2113156661376923No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCTACAACGTTCGT2380.1956931071625322No Hit
AAAGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT2120.17431486856494463No Hit
AGAGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT2110.17349262861888357No Hit
GGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT2030.16691470905039507No Hit
ACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT1910.15704782969766237No Hit
AGGGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT1800.1480031902909907No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT1770.14553647045280754No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG1750.1438919905606854No Hit
GCCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT1710.14060303077644118No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA1690.13895855088431908No Hit
CGTTCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT1660.13649183104613588No Hit
GAAGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT1640.13484735115401378No Hit
AGACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT1590.13073615142370848No Hit
GGAAGGGATCACATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGC1540.12662495169340318No Hit
GCAGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT1540.12662495169340318No Hit
GGAACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT1460.1200470321249147No Hit
TCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG1400.11511359244854834No Hit
GCAGAGACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGT1380.11346911255642621No Hit
GGATCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT1360.1118246326643041No Hit
GCAGAGAATATCAAAGCGGAAAAAATCCAAAAAAAAAAAAAAAAAAAAAA1320.10853567288005986No Hit
AAGCCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT1310.10771343293399879No Hit
GAGAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA1260.1036022332036935No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATG1250.10277999325763243No Hit
AGGCCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT1250.10277999325763243No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGGG156.876237E-494.037852
TCTAGGG156.876237E-494.037852
GACCAGG156.876237E-494.037851
AGCTAGG156.876237E-494.037851
ATCAGGG254.882877E-794.037842
TAGCAGG201.8276069E-594.037841
AATGCGG353.5470293E-1094.037841
GTTAAGG550.094.037841
GGACAGG353.5470293E-1094.037841
TACAAGG353.5470293E-1094.037841
CGGGTAT156.887493E-493.9991765
GGCAGTC156.887493E-493.9991767
GGTCGGA254.894846E-793.9991767
GGGTAAT156.887493E-493.9991766
AGGGTGC254.894846E-793.9991765
CTTGGTC156.887493E-493.9991768
CAGGGAC550.093.9991764
TCGGATG254.894846E-793.9991769
TCTGTGC301.3160388E-893.9991769
TTGGTCA156.887493E-493.9991769
Produced by FastQC (version 0.11.5)