FastQCFastQC Report
Mon 27 Feb 2023
SRR3128992.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128992.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123568
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC15621.2640813155509518No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG14711.1904376537614916No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG8750.7081121325909621No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5730.46371228797099573No Hit
CGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT4560.3690275799559757No Hit
ACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG3170.25653890975009713No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA3040.2460183866373171No Hit
AAAAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA2900.23468859251586172No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTACAACGT2820.22821442444645865No Hit
AGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT2730.22093098536838016No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCTACAACGTTCGT2630.21283827528162633No Hit
AAAACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT2580.20879192023824938No Hit
AGAGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT2260.18289524796063708No Hit
ACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT2250.18208597695196166No Hit
GAAGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT2140.17318399585653244No Hit
AAAGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT2140.17318399585653244No Hit
GGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT2080.16832836980448013No Hit
GCCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT1970.1594263887090509No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT1930.15618930467434933No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG1770.1432409685355432No Hit
GCAGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT1730.14000388450084164No Hit
GCAGAGACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGT1670.13514825844878933No Hit
CGTTCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT1650.13352971643143854No Hit
GGAAGGGATCACATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGC1650.13352971643143854No Hit
AGGCCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT1590.12867409037938624No Hit
AAGACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT1570.12705554836203548No Hit
GCAGAGAATATCCTGTCTCTTATACACATCTGACGCTACAACGTTCGTAT1520.12300919331865855No Hit
AGACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT1490.12058138029263239No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA1490.12058138029263239No Hit
GGAACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT1460.11815356726660624No Hit
GGAAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA1450.11734429625793086No Hit
TCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG1380.11167939919720317No Hit
GCAGAGAATATCAAAGCGGAAAAAATCCAAAAAAAAAAAAAAAAAAAAAA1350.10925158617117699No Hit
GAATCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT1340.1084423151625016No Hit
AATGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT1320.10682377314515085No Hit
AGATCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT1320.10682377314515085No Hit
AGGGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT1270.10277741810177392No Hit
GAGAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA1250.10115887608442314No Hit
ACACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT1240.10034960507574776No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGCGG254.873491E-794.069671
GAGTAGG550.094.0696641
ATTACGG201.8246528E-594.0696641
ATAGCGG353.5288394E-1094.0696641
TAGCAGG353.5288394E-1094.0696641
AATGCGG550.094.0696641
GGTATGG201.8246528E-594.0696641
ATAATGG156.867253E-494.069661
GCTATGG156.867253E-494.069661
CGTTCTG301.3093086E-894.069661
GTTAAGG301.3093086E-894.069661
AACTAGG156.867253E-494.069661
GACGAGG301.3093086E-894.069661
AGTACGG156.867253E-494.069661
GCACTGG156.867253E-494.069661
TAGTAGG301.3093086E-894.069661
AGGGCAA500.094.0315865
ACTGGGT254.885278E-794.0315863
GGAGCGA201.8283277E-594.031587
AATTTCG156.8783213E-494.031589
Produced by FastQC (version 0.11.5)