FastQCFastQC Report
Mon 27 Feb 2023
SRR3128991.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128991.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1832809
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127900.6978359447165526No Hit
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC35510.19374632053858312TruSeq Adapter, Index 27 (100% over 24bp)
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG35400.19314614888949147TruSeq Adapter, Index 27 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG33700.18387076885807524TruSeq Adapter, Index 27 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT48850.078.5970841
TAGGGCA7800.075.558564
CGTAGGG5800.072.348622
AGTAGGG28700.072.283482
AGGGCAT12750.071.8715065
ATGCGGG10200.069.798332
AGTAAGG11200.069.4884341
GGTAAGG10050.069.46121
TAGAGGG31200.069.211652
CGAAGGG20150.067.388492
TAGTAGG7450.067.111391
AGGGATG23350.066.615215
TATAGGG15950.066.510482
ATAGGGC9900.066.198463
GTAGGGA12900.066.154163
GTAGGGC8300.065.894353
AAGGGGC54450.065.80884
GAATAGG10250.065.8049851
GGATAGG12150.065.219821
AAGAGGG64600.064.883992
Produced by FastQC (version 0.11.5)