FastQCFastQC Report
Mon 27 Feb 2023
SRR3128990.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128990.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1875701
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120970.6449322146760065No Hit
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC39040.2081355184008539TruSeq Adapter, Index 27 (100% over 24bp)
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG37400.19939212059917866TruSeq Adapter, Index 27 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG35940.19160836401963854TruSeq Adapter, Index 27 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT44100.077.992361
CGTAGGG5200.075.063342
ATAGGGC9950.074.629273
AGATAGG8750.072.0559841
GTAGGGA12100.070.690533
GGTAAGG11250.070.681781
ATAGGGA13450.069.884833
AGTAGGG29750.069.711552
GAATAGG10750.068.717041
TAGGGCA8500.068.561134
ATAGAGG13250.068.535361
TATAGGG14100.068.373512
AGGGCAT14300.068.360075
AGGGTAG7750.067.3126145
AGGGATG23050.067.081095
TAGAGGG30600.067.006692
GTAGGGC7450.066.8689963
CGAAGGG20200.066.816492
AAGGGTA13100.066.729344
AGGGTAC9950.066.599545
Produced by FastQC (version 0.11.5)