FastQCFastQC Report
Mon 27 Feb 2023
SRR3128989.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128989.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1947618
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126510.6495626965862916No Hit
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC39050.20050133034301387TruSeq Adapter, Index 27 (100% over 24bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG38810.19926905584154594TruSeq Adapter, Index 27 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG38310.19670181729682107TruSeq Adapter, Index 27 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT49700.077.719871
ATAGGGC10100.071.658043
TAGGGCA7500.070.8080754
GGATAGG12750.070.480421
AGTAGGG30900.070.418722
GGTAAGG12550.069.729171
AGTAAGG12150.069.314171
TAGTAGG8650.068.5330051
GAATAGG10750.068.2749561
AGGGATG22750.067.964185
GTAGGGA13200.067.6464843
TAGAGGG33800.067.4358142
AAGGGTA13700.067.235884
CGTAGGG5250.067.137872
TATAGGG16600.066.5312042
AGGGCAT13500.066.1432345
AAGGGGC57650.065.705444
GTAGGGC7250.064.822773
ATAGAGG13700.064.562831
ATAACGG950.064.382091
Produced by FastQC (version 0.11.5)