FastQCFastQC Report
Mon 27 Feb 2023
SRR3128988.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128988.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2107038
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146390.6947667768687608No Hit
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG39520.18756187596047152TruSeq Adapter, Index 23 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC38380.18215143723084254TruSeq Adapter, Index 20 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG35730.16957454018389798TruSeq Adapter, Index 23 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC31830.15106514452990408No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGTAC13150.083.264115
CGTTTTT57450.080.890661
CGTAGGG5100.076.7565162
TAGGGCG3650.076.237124
GAGGGTA13050.075.172714
AGGGATG38550.073.0180365
TAGGGCA10750.071.513254
AAGGGAT37550.071.090874
TAGGGTA7000.070.745474
AGGGCAT18050.070.5536965
AAGGGTA17850.070.283084
TAAGGGA17400.069.9323043
AGAGGGA48100.069.912023
AGTAGGG35700.069.226192
AGGGAAT23150.069.219915
ACGGGTA2950.068.747014
TAGAGGG37100.068.647892
GGGTACC17350.067.71246
AAGAGGG70350.067.0414352
AGTAAGG14650.066.9850541
Produced by FastQC (version 0.11.5)