FastQCFastQC Report
Mon 27 Feb 2023
SRR3128987.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128987.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2142075
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141550.6608078615361274No Hit
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG40890.1908896747312769TruSeq Adapter, Index 23 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC40240.18785523382701352TruSeq Adapter, Index 20 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG38030.177538134752518TruSeq Adapter, Index 23 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC32790.15307587269353315No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT57200.078.893441
AGGGATG37800.075.593135
ACGGGTA2450.074.811634
AGGGTAC12750.074.089465
AAGGGTA17900.073.514934
CGTAGGG6100.072.465662
GAGGGTA13500.072.410264
ATAAGGG24700.070.633572
TAGGGCA9100.070.237344
TAGAGGG34650.069.62232
GGGTACC17700.069.3007356
TAAGGGA18700.069.113313
AGTAAGG15050.069.099371
AGGGTAT12900.068.856145
AGGGAAT26700.068.823395
ATAGGGA20300.068.7592243
ATAGCGG4450.068.734011
TAGGGTA6300.068.630614
AGGGTAA9800.068.577335
AGAGGGA49350.066.948173
Produced by FastQC (version 0.11.5)