FastQCFastQC Report
Mon 27 Feb 2023
SRR3128986.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128986.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2238916
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147790.660096225137522No Hit
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG41380.1848215833019193TruSeq Adapter, Index 23 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC40550.1811144321627073TruSeq Adapter, Index 20 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG38100.17017163663129836TruSeq Adapter, Index 23 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33760.15078725597565965No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGTAC13550.080.811055
CGTTTTT57100.079.67641
CGTAGGG5550.078.747062
GAGGGTA14400.076.6936654
ACGGGTA2650.076.256474
AGGGATG39500.073.88375
AGTAAGG13900.073.448721
TAGGGTA6400.073.43014
TAAGGGA18500.073.1601943
GGGTACC17250.072.468066
AAGGGAT40200.071.6619344
AGATAGG12050.071.450191
AGGGAAT25850.071.083755
AGGGCAT18250.071.072295
TAGAGGG35500.069.895622
AAGGGTA18850.069.807294
ATAACGG2300.069.548921
AAGAGGG77850.068.63512
TATAGGG17600.068.3552252
ATGAGGG37500.068.047622
Produced by FastQC (version 0.11.5)