FastQCFastQC Report
Mon 27 Feb 2023
SRR3128985.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128985.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2557700
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247560.9679008484185009No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG35910.14039957774563083No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC33840.13230636900340148No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT103550.084.4468461
ACGGGTA4650.076.046424
TAGGGCA9850.073.236174
AGGGATG41350.070.574755
AGGGTAC14850.070.5686345
GAGGGTA17750.070.125514
AGTAGGG43000.069.7363362
ATAGGGA22000.069.65163
GTAGGGA18900.069.600583
ATAGGGC13050.069.368323
AGGGTAT16400.069.3433465
AAGGGTA20550.069.28924
CGTAGGG7500.069.151542
TAGAGGG48300.067.8434752
AAGAGGG92900.067.348152
GTAGGGC10950.066.740283
AGTAAGG18000.066.03161
AGAGGGA56400.065.8745353
TAAGGGA20200.065.821563
AAGGGGC80150.065.7671054
Produced by FastQC (version 0.11.5)