FastQCFastQC Report
Mon 27 Feb 2023
SRR3128984.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128984.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2656588
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241670.9097007138479885No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG38260.1440193210238095No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC37150.13984102916974706No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT103000.083.687931
AGGGATG41300.073.056795
TAGGGCA11650.071.807494
CGTAGGG7050.071.36732
ATAGGGC15550.070.11863
GGTAAGG20600.068.750181
ACGGGTA4200.068.2584154
AGGGAAT24850.067.895935
AGGGTAC15050.067.7639855
AGTAGGG45150.067.6955642
TAGAGGG47400.067.656672
ATAGGGA23200.067.052613
TAAGGGA20800.066.88123
TAGTAGG14650.066.8036651
AAGGGAT41200.066.389824
AAGGGTA21400.066.3237154
ATAGAGG17600.066.300011
AAGAGGG99750.065.902052
ATAAGGG35100.065.777752
TATAGGG22000.065.617592
Produced by FastQC (version 0.11.5)