FastQCFastQC Report
Mon 27 Feb 2023
SRR3128983.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128983.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2831857
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248910.878963874235175No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG41210.14552288480668338No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC38640.13644756779738526No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT105100.083.937141
ATAGGGC14150.071.409223
GAGGGTA19550.070.4357454
AGGGATG44800.070.178655
AGGGTAC17500.069.5534445
AAGGGTA24350.067.93854
AGGGTAT16850.067.7740255
TAGGGCA11250.067.6764
GGTAAGG20700.067.5059051
TAAGGGA23200.067.457233
TAGGGTA8650.067.371754
AAGGGAT44950.067.019414
AGTAGGG47250.066.7410352
AAGAGGG110250.066.584742
TAGAGGG54300.066.384672
AGATAGG17600.066.297111
GTACGGG7250.066.120242
GTAGGGA19100.065.9437563
ATAGGGA23650.065.7762453
ATAGCGG7200.065.346621
Produced by FastQC (version 0.11.5)