FastQCFastQC Report
Mon 27 Feb 2023
SRR3128982.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128982.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2850857
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259820.9113750707243471No Hit
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC42970.1507266060696836TruSeq Adapter, Index 21 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG41580.145850879226843TruSeq Adapter, Index 15 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC32820.11512327696548792No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG30320.1063539840826811TruSeq Adapter, Index 15 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT95100.081.5034941
AGGGTAC18300.073.9560855
TAGGGCA14550.073.891124
ACGGGTA3550.070.399234
TAGAGGG49700.069.450452
AGTAAGG19100.069.405211
GAGGGTA19150.068.946094
AAGAGGG106650.068.399022
AGGGATG44000.068.353365
GAATAGG17600.068.0832441
TAAGGGA21350.067.804873
ATAGGGC17550.067.7085953
AAGGGTA24950.066.526174
AGAGGGA66800.066.495893
ATAGGGA22650.066.411443
GAGGGAT36700.066.298554
AGGGCAT23850.066.203885
ATGAGGG42450.066.0936362
AGAGGGC41300.065.650533
AGTAGGG44300.065.249492
Produced by FastQC (version 0.11.5)