FastQCFastQC Report
Mon 27 Feb 2023
SRR3128981.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128981.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2903613
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255540.8800759605360632No Hit
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC44780.15422165419427453TruSeq Adapter, Index 21 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG43710.1505365901034332TruSeq Adapter, Index 15 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC32760.1128249529121133No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG31490.1084510917949465TruSeq Adapter, Index 15 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT91650.080.824351
AGGGTAC16150.077.699375
TAGGGCA15100.073.453724
ACGGGTA3800.071.733694
AGGGATG42150.070.692035
ATAGGGC17850.070.299433
ATAGGGA22800.069.466253
AGGGCAT23700.069.009635
AAGGGTA24400.068.9559864
GAATAGG16750.068.836881
AAGGGAT43100.068.26154
AGTAAGG16550.067.6782151
AAGAGGG106750.067.3090742
TAGGGCG5550.066.8979644
TAGAGGG52700.066.8327562
AGAGGGC39300.066.251543
GAGGGTA21150.065.99714
ATGAGGG44400.065.875752
TGTAGGG21600.065.74632
GGGTACC19300.064.287336
Produced by FastQC (version 0.11.5)