FastQCFastQC Report
Mon 27 Feb 2023
SRR3128980.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128980.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3024557
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263490.8711689017598281No Hit
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC47720.1577751717028312TruSeq Adapter, Index 21 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG47250.15622122512486952TruSeq Adapter, Index 15 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC34650.11456223175823767No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG34100.11274378363509102TruSeq Adapter, Index 15 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT95100.081.4644551
AGGGTAC17500.074.115585
TAGGGCA14400.073.752574
GAATAGG18750.073.004021
TAGAGGG54450.070.424462
AAGGGTA24750.069.3025054
AGTAGGG49400.069.15742
ACGGGTA4350.069.138824
AAGAGGG114350.068.670712
ATAGGGC16700.068.661263
CGTAGGG9400.068.48952
AGTAAGG19500.068.266381
AGGGATG44450.067.8737265
ATAGGGA26450.067.869733
AGGGCAT24950.067.6185
GAGGGTA19750.067.574454
ATAGAGG21750.067.476171
AGTTAGG8250.066.139221
GTAGGGC13500.066.139093
AGAGGGC45600.065.749773
Produced by FastQC (version 0.11.5)