FastQCFastQC Report
Mon 27 Feb 2023
SRR3128979.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128979.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2739973
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222580.8123437712707389No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC36730.13405241584497365No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG32240.11766539305314322TruSeq Adapter, Index 14 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT90450.081.1419141
AGGGATG42350.072.2370455
CGTAGGG7250.072.190172
TAGGGCA13550.070.639874
ATAGGGC15100.070.570733
TAGAGGG50600.070.354122
GGTAAGG16450.069.389491
ATAGGGA24200.069.362983
GAATAGG19450.068.872041
AGTAGGG48450.067.831612
TATAGGG19550.067.531182
AAGGGAT43700.067.3280944
GTAGGGA23050.067.09593
AGATAGG17250.066.991731
GAGGGAT33800.066.681344
TAGGGAT16750.066.433984
AGAGGGC39100.066.084093
TAGGGTA10450.065.876374
AGGGTAC15500.065.7900855
AGTAAGG17250.065.624561
Produced by FastQC (version 0.11.5)