FastQCFastQC Report
Mon 27 Feb 2023
SRR3128978.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128978.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2788294
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215770.7738423566524907No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC38640.13857936071303814No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG34060.12215354621858383TruSeq Adapter, Index 14 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT84850.079.967331
ATAGGGC15150.072.2790763
AGGGTAC15550.071.628735
AGGGATG37900.071.425385
GGTAAGG16800.070.861521
TAGGGAT17750.070.164394
AGGGCAT25000.070.1193855
TAGGGCA14950.069.788054
AGTAGGG46350.069.703842
ATAGGGA25450.069.618213
TAGAGGG49500.069.448352
GTAGGGA20850.067.396053
CGTAGGG6400.066.132092
GTAGGGC12250.065.603843
ATAAGGG39500.065.600062
ATGTAGG8700.065.443311
GATAGGG43600.065.255722
AAGAGGG102200.064.8810652
AGATAGG15450.064.870961
TATAGGG20200.064.720692
Produced by FastQC (version 0.11.5)