FastQCFastQC Report
Mon 27 Feb 2023
SRR3128977.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128977.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2988493
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223580.7481362680120047No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC40150.13434864997174162No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG35030.11721626920324056TruSeq Adapter, Index 14 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT89700.080.51761
AGGGTAC16550.072.407965
GGTAAGG17700.070.7103961
ATAGGGA26000.070.310533
AGTAGGG48150.070.174082
GACCGAT2500.069.55148
AGGGATG41500.068.9625555
GGATAGG21150.068.074731
TAGGGCA14050.067.899074
GAGGGAT36000.067.6194154
ATAGGGC16850.067.493133
CGTAGGG8550.067.056032
TAGAGGG53700.067.034542
AAGAGGG109800.066.682092
ATGTAGG9150.066.335121
TAGGGTA10500.066.2394264
AGTAAGG18000.066.133711
AGATAGG16600.065.4754941
TAGGGAT17050.065.3232964
AGGGCAT22900.065.258315
Produced by FastQC (version 0.11.5)