FastQCFastQC Report
Mon 27 Feb 2023
SRR3128976.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128976.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2283861
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256721.1240614030363494No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC89510.3919240269000609No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG81690.3576837644672771No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG58160.25465647865610036No Hit
CGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT55470.24287817866323738No Hit
AGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT28300.12391297018513824No Hit
GGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT26180.11463044379671093No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT97100.084.445621
ACGGGTA2850.076.0843354
CGTAGGG6750.074.724372
AGGGAAT22900.073.4661255
AGTAAGG12950.071.728741
AGGGATG34500.071.375935
GGTAAGG12000.069.548661
ATAGAGG12050.069.260081
ATAGGGC11850.069.2170643
AGAGGGA46800.069.1979453
GTAGGGC8150.068.8290253
AGTACGG2900.068.2885741
TAGGGCA11100.067.948414
GAGGGAT27850.067.873694
AGTAGGG33150.067.6870652
TAGAGGG30800.067.647832
TAAGGGA18550.067.595853
GAATAGG12250.067.3594741
AAGGGTA13350.067.089514
ATAAGGG26400.066.780552
Produced by FastQC (version 0.11.5)