FastQCFastQC Report
Mon 27 Feb 2023
SRR3128975.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128975.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2328163
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249871.0732495963555817No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC95730.4111825503626679No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG85370.36668394781636854No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG59480.25548039376968024No Hit
CGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT57250.24590202661927021No Hit
AGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT29600.1271388644179982No Hit
GGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT26800.11511221508116054No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT94300.082.828021
CGTAGGG6200.076.60362
ATAGGGC12050.074.492743
ATAGGGA19200.073.432383
AGGGAAT22350.072.9658365
TAGGGCA9350.071.374714
AGGGATG33550.070.4600755
TATAGGG15700.070.386232
AGTAAGG12850.068.8391651
GAATAGG12250.068.369871
AATAGGG29650.068.355282
AAGAGGG77700.068.1454542
GTAGGGC8000.068.145253
TAAGGGA18650.068.0381553
GGGCGAT9100.067.6546256
TAGAGGG35600.067.630012
TAGGGAT14550.067.5073244
ATGAGGG32100.066.653942
GAGGGAT26500.066.50484
AAGGGTA14650.066.404934
Produced by FastQC (version 0.11.5)