FastQCFastQC Report
Mon 27 Feb 2023
SRR3128974.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128974.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2425494
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259101.0682359964609272No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC103120.4251505054228128No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG92160.37996383417151314No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG66940.2759850158359493No Hit
CGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT61100.25190744648306695No Hit
AGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT30920.1274791856834113No Hit
GGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT27820.1146982841433539No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT97450.083.446951
CGTAGGG6150.077.948752
AGTAAGG15550.073.842781
GAATAGG13250.072.809251
ATAGAGG14150.072.501781
TAGGGCA11200.071.756664
ATAGGGC13350.070.409773
ATAGGGA19050.070.3127443
AGGGATG36200.069.972685
ACGGGTA2650.069.1676254
AGTAGGG33350.069.053292
TAGAGGG37650.068.90622
AAGAGGG81050.068.830642
AAGGGCA27350.068.3927154
AGGGAAT23200.068.263765
AATAGGG32800.067.918592
GTAGGGA15850.067.903213
AGGGTAG8350.067.537215
AGAGGGC31250.067.076283
GAGGGAT27200.066.869214
Produced by FastQC (version 0.11.5)