FastQCFastQC Report
Mon 27 Feb 2023
SRR3128973.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128973.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2810715
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245690.8741192187752939No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG45280.16109779895862797No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC41560.14786273243640852No Hit
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG28470.10129095265795358No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT108050.082.364021
ACGGGTA2950.075.144094
AGGGTAC14800.073.663245
AGGGATG39100.072.5913245
ATAGGGC14650.072.115623
TAGGGCA12450.071.22094
GAATAGG15900.069.741511
TAGAGGG49100.068.874212
AGTAAGG19500.068.723361
AGTAGGG45000.068.23192
ATAGCGG5050.068.210641
AAGGGAC29350.068.136024
ATAGGGA24600.067.1045463
AGGGCAT21300.067.068495
AAGGGTA18100.066.968954
TAAGGGA26950.066.67843
CGTAGGG7500.066.659742
AAGGGAT45750.066.28864
AAGAGGG102550.066.044662
TAGGGAT17500.065.761354
Produced by FastQC (version 0.11.5)