FastQCFastQC Report
Mon 27 Feb 2023
SRR3128972.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128972.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2866406
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242150.844786118923837No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG48830.17035269951290918No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC45590.1590493461149607No Hit
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG29860.10417226310578474No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT112550.083.938221
TAGGGCA12250.075.57914
GAATAGG17600.072.1759641
CGTAGGG8000.071.117932
AGTAGGG46550.070.504932
AGGGATG37150.070.21115
ATAGGGC15600.070.194473
GTACGGG5950.069.5423662
ATAGGGA25550.069.34613
TAAGAGG22800.067.8894961
AGGGTAC16150.067.512955
ACGGGTA2950.066.911214
TAGGGTA9100.066.6223454
TAAGGGA25700.066.564073
TAGGGAT16600.066.532124
TATAGGG23050.066.501352
TAGAGGG52150.066.4502942
AGTAAGG19050.066.4352951
AAGAGGG102350.066.384052
ATAAGGG40850.066.069892
Produced by FastQC (version 0.11.5)