FastQCFastQC Report
Mon 27 Feb 2023
SRR3128971.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128971.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2993962
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244720.8173784436809819No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG50540.16880641771672453No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC48560.1621931073273475No Hit
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG31820.10628057403534179No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT110550.083.826861
ATAGCGG5700.075.10041
GAATAGG18200.074.438371
AGGGATG38250.072.246245
TAGGGCA14900.072.231374
ATAGGGC18200.071.7877043
AGGGTAC17200.071.588335
TAGAGGG54400.070.322592
ATAGGGA25950.068.459083
CGTAGGG8650.068.457942
AGTAGGG48800.068.184052
ACGGGTA4000.068.146674
AAGAGGG104350.068.0971152
TAGGGTA9050.067.510514
AAGGGAC31550.067.33114
TAGGGAT17750.066.988284
TATAGGG24150.066.554642
ATAAGGG42500.066.348592
AGGGTAT13550.066.246585
AGATAGG19050.066.178291
Produced by FastQC (version 0.11.5)