FastQCFastQC Report
Mon 27 Feb 2023
SRR3128970.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128970.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177885
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC39172.201984428141777No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG30521.7157152092644123No Hit
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG17250.969727633021334No Hit
CGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT13370.7516091857098687No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6410.3603451668212609No Hit
AGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT6200.34853978694100124No Hit
ACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG5200.2923236922730978No Hit
AAATCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT5080.2855777609129494No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT4380.246226494645417No Hit
ACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT4380.246226494645417No Hit
AAAGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT4200.23610759760519434No Hit
GGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT3960.22261573488489755No Hit
AGAGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT3910.21980493015150235No Hit
GCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT3830.21530764257807009No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT3560.20012929701773616No Hit
GGAACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT3280.1843887905107232No Hit
GAAACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT3280.1843887905107232No Hit
TCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG3180.17876718104393288No Hit
GAAGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT3180.17876718104393288No Hit
AATGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT3120.17539421536385869No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCC2750.15459426033673443No Hit
AAGCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT2620.14728616802990696No Hit
AGAACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT2540.1427888804564747No Hit
AAATGATACGGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATG2470.13885375382972145No Hit
GCAGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT2420.13604294909632628No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGT2350.13210782246957303No Hit
AAAACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT2340.13154566152289401No Hit
CGTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTC2220.12479973016274559No Hit
AAGACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT2170.12198892542935043No Hit
ATCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT2080.1169294769092391No Hit
AGGCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT2080.1169294769092391No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT2070.11636731596256007No Hit
AGGACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT2010.11299435028248588No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA1950.10962138460241166No Hit
ATAGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT1930.1084970627090536No Hit
GCAGAGAATATCAAAGCGGAAAAAATCCAAAAAAAAAAAAAAAAAAAAAA1920.10793490176237457No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA1830.10287545324226327No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGG156.811254E-494.280881
ATGACGG254.817575E-794.280881
TAGTGGG156.811254E-494.280881
ATGTAGG409.094947E-1294.2808761
GCGTAGG201.806633E-594.2808761
TAGGGCG201.8117003E-594.227744
GAGGGTA201.8117003E-594.227744
GAGGACC156.826542E-494.227734
GCACGGG156.826542E-494.227732
ACGGGTA254.8337824E-794.227734
CAGGGTA254.8337824E-794.227734
GGGTATA254.907306E-793.989326
GGGTACG650.093.989326
GGATGCG156.895656E-493.989317
GGGTCAA156.895656E-493.989316
GGTATAG156.895656E-493.989317
CGGGAAT1550.090.9574055
TAGTAGG750.087.995481
GTACGGC700.087.2757958
ATGGGCC650.086.979454
Produced by FastQC (version 0.11.5)