FastQCFastQC Report
Mon 27 Feb 2023
SRR3128969.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128969.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183777
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC42202.296261229642447No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG32901.7902131387496805No Hit
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG17640.9598589594998286No Hit
CGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT14050.7645135136605777No Hit
AGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT6480.3526012504285085No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6190.33682125619636843No Hit
AAATCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT5700.3101585073213732No Hit
ACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG5570.30308471680351734No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT4730.25737714730352546No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA4510.24540611719638478No Hit
AGAGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT4500.24486197946424199No Hit
ACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT4470.2432295662678137No Hit
AAAGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT4450.2421412908035282No Hit
GGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT4430.24105301533924264No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTTGTCCC3920.2133019909999619No Hit
GCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT3910.21275785326781918No Hit
GAAGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT3760.20459578728567773No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT3700.2013309608928212No Hit
AGAACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT3290.17902131387496803No Hit
GGAACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT3280.17847717614282527No Hit
GAAACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT3270.1779330384106825No Hit
TCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG3200.1741240742856832No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCC3180.17303579882139766No Hit
AATGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT3060.16650614603568456No Hit
AAAACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT2630.14310822355354588No Hit
AAGCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT2630.14310822355354588No Hit
ATAGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT2590.14093167262497483No Hit
AAGACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT2490.13549029530354725No Hit
GCAGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT2450.13331374437497617No Hit
GCAGAGAATATCAAAGCGGAAAAAATCCAAAAAAAAAAAAAAAAAAAAAA2380.1295047802499769No Hit
GGAGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT2380.1295047802499769No Hit
AAATGATACGGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATG2270.12351926519640652No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGT2200.11971030107140719No Hit
AGGACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT2150.11698961241069339No Hit
CGTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTC2140.11644547467855064No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT2110.11481306148212235No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATG2070.11263651055355131No Hit
ATCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT2040.11100409735712305No Hit
AGGCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT1960.10665099549998094No Hit
GAGACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT1950.1061068577678382No Hit
AGGGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT1910.10393030683926716No Hit
GCAGAGACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGT1880.10229789364283887No Hit
TGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT1870.10175375591069613No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG156.856327E-494.1258851
ATAGAGG1150.094.1258851
CGGGTGA156.893543E-493.9978265
GGAGAGT201.833983E-593.9978267
CGCGGGC156.893543E-493.9978263
GGGTACC156.893543E-493.9978266
GGATCGG301.3198587E-893.9978268
AGGGTCC254.9053233E-793.9978265
ACGGGAT156.893543E-493.9978264
CGGGGAT450.093.9978265
TCGTGTA156.893543E-493.9978269
TGGGGTA156.893543E-493.9978265
TAGGGCG201.833983E-593.9978264
ATTGGGC156.893543E-493.9978263
AGTAGTG156.893543E-493.9978267
AGGGCGT201.833983E-593.9978265
TATCGCG156.893543E-493.9978269
GTAAAGG900.088.896671
AGGGCCC3450.088.548675
GTAGAGG650.086.885441
Produced by FastQC (version 0.11.5)