FastQCFastQC Report
Mon 27 Feb 2023
SRR3128968.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128968.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188344
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC43922.3319033258293334No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG32591.7303444760650724No Hit
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG19351.0273754406830056No Hit
CGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT14670.7788939387503716No Hit
AGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT6370.338210933186085No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6070.322282631780147No Hit
AAATCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT5490.29148791572866667No Hit
ACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG5410.28724036868708325No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT4990.2649407467187699No Hit
ACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT4960.2633479165781761No Hit
AGAGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT4360.23149131376629994No Hit
AAAGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT4360.23149131376629994No Hit
GGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT4230.2245890498237268No Hit
GCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT4020.21343923883957017No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA3960.21025357855838253No Hit
TCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG3780.20069659771481968No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT3760.1996347109544238No Hit
GAAACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT3710.19697999405343414No Hit
GAAGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT3610.1916705602514548No Hit
GGAACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT3550.18848489997026718No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTTGTCCC3180.16883999490294355No Hit
AATGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT3090.16406150448116213No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCC2990.1587520706791828No Hit
AGAACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT2970.15769018391878692No Hit
AAGCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT2630.1396381089920571No Hit
AAGACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT2600.13804527885146328No Hit
AAATGATACGGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATG2600.13804527885146328No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGT2560.13592150533067154No Hit
AAAACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT2550.1353905619504736No Hit
GCAGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT2530.13432867519007774No Hit
GCAGAGAATATCAAAGCGGAAAAAATCCAAAAAAAAAAAAAAAAAAAAAA2450.13008112814849423No Hit
GGAGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT2360.12530263772671282No Hit
AGGCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT2250.11946226054453553No Hit
CGTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTC2180.11574565688314997No Hit
ATCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT2090.11096716646136855No Hit
ATAGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT2070.10990527970097269No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT2020.10725056279998302No Hit
AGGACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT2010.10671961941978508No Hit
AGACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT1930.10247207237820159No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAAGG353.5652192E-1094.0443651
TGAACGG201.8295967E-594.0443651
TGAAAGG700.094.0443651
TGTTAGG201.8295967E-594.0443651
AGTACGG201.8295967E-594.0443651
GGTAAGT156.880256E-494.044361
GGATGCG201.8344308E-593.994427
GTAGGGT353.5652192E-1093.994423
CAGGGCG254.906851E-793.994424
CAGGGAG353.5652192E-1093.994424
CGTAGGG450.093.994422
CGGGATC254.906851E-793.994425
GGATTCA201.8344308E-593.994428
GGCCGAT156.8948034E-493.9944157
TAGGGTA156.8948034E-493.9944154
GTAAGTT156.8948034E-493.9944152
GTACGGG301.3204044E-893.9944152
AGTAAGG1050.089.566061
AGGGCGA800.088.1197745
GGGCGAT600.086.1615456
Produced by FastQC (version 0.11.5)