FastQCFastQC Report
Mon 27 Feb 2023
SRR3128967.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128967.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1530250
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131990.8625388008495345No Hit
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC69080.45142950498284595Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG59780.39065512171213856No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG48510.3170070249959157No Hit
CGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT32190.21035778467570657No Hit
AGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT20830.13612154876654142No Hit
GGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT20390.13324620160104558No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT50450.081.138771
AAGGGTA10400.078.435274
AGGGATG23300.076.442015
ATAGAGG9500.075.4553151
AGATAGG6750.074.756661
AGGGAAT18200.074.365235
GGTAAGG8000.074.276331
ATAGCGG3400.073.513451
GAATAGG8200.072.464711
CGGGAAT3700.072.3972245
TAAGGGA14050.071.818473
TAAGCGG3550.071.735691
ATAGGGC8300.071.011823
CGTAGGG4000.070.727762
ACGGGAT3350.070.3758854
AGGGTAT8300.070.208945
AGAGGGA31550.070.09263
AAGGGAT22950.070.0600364
GTAGGGC7600.069.486923
TATAGGG8850.069.262592
Produced by FastQC (version 0.11.5)